Microfluidic Chips for Formulation of Silica Nanoparticles and Enzyme Immobilization

The unique micromixing environment of microfluidic technology provides new opportunities to apply known nanoparticle formulation techniques for higher reaction rates and better product quality. Novel methods to formulate silica nanoparticles and immobilize Rhizomucor miehei lipase on these particles...

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Bibliographic Details
Published in:Chemical engineering & technology 2021-08, Vol.44 (8), p.1423-1431
Main Authors: Ling, Fiona W. M., Abdulbari, Hayder A., Chin, Sim-Yee
Format: Article
Language:English
Subjects:
Chip formation
Enzyme immobilization
Immobilization
Lipase
Lipases
Microfluidic chips
Microfluidics
Micromixing
Nanoparticles
Silicon dioxide
System effectiveness
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Summary:The unique micromixing environment of microfluidic technology provides new opportunities to apply known nanoparticle formulation techniques for higher reaction rates and better product quality. Novel methods to formulate silica nanoparticles and immobilize Rhizomucor miehei lipase on these particles in a microflow environment are introduced. The quality of the formulated nanoparticles from the microfluidic reactor was compared with those produced in the traditional bench‐scale method. The results demonstrate that the smaller silica nanoparticles were produced from the microfluidic reactor with higher dispersity compared to nanoparticles obtained from the bench‐scale. Immobilization of lipase is found to be more effective using silica nanoparticles synthesized in microflow systems as the carriers. Highly monodispersed silica nanoparticles were successfully synthesized in a droplet‐based microflow system. The produced nanoparticles were immobilized with Rhizomucor miehei lipase via physical adsorption in a microreactor, achieving high lipase affinity on the carriers with high immobilization yield within a short residence time.
ISSN:0930-7516
1521-4125
DOI:10.1002/ceat.202100098