Genetic diversity and the presence of circular plasmids in Bacillus cereus isolates of clinical and environmental origin

The diversity of 61 Bacillus cereus strains isolated from different clinical specimens, food including raw milk and milk products, and water was evaluated. PFGE analysis could discriminate 61 distinct pulsotypes with similarity levels from 25 to 82%, which were divided into 13 clonal complexes. The...

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Bibliographic Details
Published in:Archives of microbiology 2021-08, Vol.203 (6), p.3209-3217
Main Authors: Cerar Kišek, Tjaša, Pogačnik, Nežka, Godič Torkar, Karmen
Format: Article
Language:English
Subjects:
Bacillus cereus
Biochemistry
Biomedical and Life Sciences
Biotechnology
Cell Biology
Clinical isolates
Ecology
Food
Food sources
Genetic diversity
Life Sciences
Microbial Ecology
Microbiology
Milk
Milk products
Original Paper
Plasmids
Similarity
Strains (organisms)
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Summary:The diversity of 61 Bacillus cereus strains isolated from different clinical specimens, food including raw milk and milk products, and water was evaluated. PFGE analysis could discriminate 61 distinct pulsotypes with similarity levels from 25 to 82%, which were divided into 13 clonal complexes. The similarity between clonal complexes was at least 40%. Clinical strains were divided into 10 clonal complexes, while the strains, isolated from milk, food and water were included in 9, 6 and 6 clonal complexes, respectively. Three clonal complexes were dominated by clinical isolates, while they were absent in two complexes. Bacterial isolates from food, being a probable source of alimentary toxoinfection, showed low similarity to isolates from stool specimens. The isolates from both sources were classified together in only 4 out of 13 clonal complexes. The large circular and linear plasmids with the sizes between 50 and 200 kb were detected in 24 (39.3%) and 14 (23%) B. cereus strains, respectively. Thirteen (21.3%) strains contained only one plasmid, two plasmids were found in 6 (9.8%) of strains, and three or more plasmids were obtained in 5 (8.2%) of tested strains. The plasmids were confirmed in 30.8% and 40% of isolates from clinical specimens and food and milk samples, respectively. No clear correlation between the PFGE profiles, the source as well as plasmid content among all tested strains was observed.
ISSN:0302-8933
1432-072X
DOI:10.1007/s00203-021-02302-w