Liquid Chromatographic Method for Simultaneous estimation of Thiocolchicoside and Etoricoxib from Tablet formulation

A new simple, reproducible and efficient liquid chromatographic method (RP-HPLC) was developed for simultaneous estimation of thiocolchicoside and etoricoxib for tablet formulation. Formulation containing thiocolchicoside and etoricoxib is used as analgesic. Separation was achieved by Nucleosil (4.6...

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Bibliographic Details
Published in:Asian Journal of Pharmaceutical Analysis 2021-06, Vol.11 (2), p.118-122
Main Authors: Gulhane, Chaitanya A., Panchale, Wrushali A., Manwar, Jagdish V., Bakal, Ravindra L.
Format: Article
Language:English
Subjects:
Accuracy
Calibration
Chromatography
Drugs
Literature reviews
Methods
Pharmaceutical industry
Pharmaceuticals
Pharmacy
Reagents
Retention
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Summary:A new simple, reproducible and efficient liquid chromatographic method (RP-HPLC) was developed for simultaneous estimation of thiocolchicoside and etoricoxib for tablet formulation. Formulation containing thiocolchicoside and etoricoxib is used as analgesic. Separation was achieved by Nucleosil (4.6mm I.D × 250 mm) C18 column with mobile phase consist of Acetonitrile: water (0.05% Orthophosphoric acid V/V) in the ratio 25:75 at flow rate 0.7ml . The detection was carried out at 220nm. The retention time of thiocolchicoside and etoricoxib was found to be 3.75 min and 6.13 min respectively. Linearity of THC and ETR was found to be in the range of 2-10µg/mL and 30-150µg/mL. Percent recovery obtained for THC and ETR were 99.98% and 99.69% respectively. The method was validated as per ICH guidelines.
ISSN:2231-5667
2231-5675
DOI:10.52711/2231-5675.2021.00020