Effect of Allelic Variants of Aromatic Alcohol Dehydrogenase CADim on Micromorphological and Chemical Tissue Indices in the Spring Bread Wheat Triticum aestivum L

• Published in: Applied biochemistry and microbiology 2021, Vol.57 (4), p.521-532
• Main Authors: Konovalov, A. A., Karpova, E. V., Shundrina, I. K., Razmakhnin, E. P., Eltsov, I. V., Goncharov, N. P.
• Format: Article
• Language: English
• Subjects: Alcohol dehydrogenase; Biochemistry; Biomedical and Life Sciences; Biotechnology; Bread; Carbonyl compounds; Carbonyl groups; Carbonyls; Chemical composition; Chlorophyll; Cinnamon; Dehydrogenase; Dehydrogenases; Fluorescence; Gene polymorphism; Genotype & phenotype; Genotypes; Herbivores; Heredity; Homozygotes; Life Sciences; Lodging; Medical Microbiology; Microbiology; Monomers; Photosynthesis; Polymorphism; Seedlings; Wheat
• ISSN: 0003-6838; 1608-3024
• DOI: 10.1134/S0003683821040086

The inheritance of allelic variants of aromatic alcohol dehydrogenase CAD im (CAD intermediate) and their effect on the severity of plant traits in spring bread wheat (fluorescence of seedling slices, micromorphology, and chemical composition of tissues) were studied. The plants with contrast CAD im genotypes (homozygotes +/+ and –/–) were used to study the tissue autofluorescence, micromorphology, and chemical composition. The thickness of the straw walls in the CAD im– genotype is higher than that in the CAD im+ genotype, which can affect the resistance of wheat plants to lodging. Differences in the chlorophyll content, particularly, in the ratio of chlorophylls A and B , were observed; this probably affects photosynthesis. An increased content of carbonyl groups in the CAD im+ genotype, as well as differences in the cinnamon monomers of lignin, were found. It follows from these results that CAD im+ and CAD im– allelic variants have a significant effect on a number of plant traits and, consequently, that the polymorphism at the CAD im locus is functional, which enables its use in breeding and biotechnology.