Tailoring lab-on-fiber SERS optrodes towards biological targets of different sizes

•Three different types of SERS substrates were fabricated by nanosphere lithography.•Performances were compared against different bio-targets (molecule, protein, cell).•A readout system for SERS optrode operation, via optical fiber, was optimized. In this paper, we studied the influence of the geome...

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Bibliographic Details
Published in:Sensors and actuators. B, Chemical Chemical, 2021-07, Vol.339, p.129321, Article 129321
Main Authors: Managò, Stefano, Quero, Giuseppe, Zito, Gianluigi, Tullii, Gabriele, Galeotti, Francesco, Pisco, Marco, De Luca, Anna Chiara, Cusano, Andrea
Format: Article
Language:English
Subjects:
Biosensors
Diameters
Electric fields
Erythrocytes
Finite element method
Lab-on- fiber optrode
Nanospheres
Numerical analysis
Optical fibers
Optical fibre sensor
Optical sensors
Raman spectra
Self-assembly
Self-assembly nanosphere lithography
Serum albumin
Steric hindrance
Substrates
Surface-enhanced Raman spectroscopy
Three dimensional analysis
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Summary:•Three different types of SERS substrates were fabricated by nanosphere lithography.•Performances were compared against different bio-targets (molecule, protein, cell).•A readout system for SERS optrode operation, via optical fiber, was optimized. In this paper, we studied the influence of the geometrical configuration of surface-enhanced Raman scattering (SERS)-active self-assembling gold nanostructures on the interaction with biological targets of different sizes, aiming at large-scope SERS lab-on-fiber optrodes composed of highly efficient SERS substrates integrated onto optical fibre tips. By using nanosphere lithography, we fabricated three types of highly ordered and reproducible SERS-active substrates. To correlate the SERS response to the steric hindrance of the biological target, we experimentally analysed and compared the SERS spectra of three representative biological probes, i.e., ultralow-molecular-weight molecules of biphenyl-4-thiol (BPT, small molecule, 186.27 Da), bovine serum albumin (BSA, medium molecule, 66.5 kDa) and red blood cells (RBCs, diameter
ISSN:0925-4005
1873-3077
DOI:10.1016/j.snb.2020.129321