Thermal and Reused Stability of Immobilized Lipase in Carrageenan

Lipase is one kind of enzyme that is widely used as a catalyst in industrial and medical. Lipase has a great catalytic ability, but is easily affected by temperature, and is also difficult to separate at the end of the reaction. This causes most of lipase used only in one reaction cycle. Therefore a...

Full description

Saved in:
Bibliographic Details
Published in:IOP conference series. Earth and environmental science 2019-06, Vol.292 (1), p.12028
Main Authors: Wardoyo, F A, Hidayah, F F
Format: Article
Language:English
Subjects:
Carrageenan
Catalysts
Catalytic activity
Entrapment
Enzymes
High temperature
Immobilization
Lipase
Palm oil
Thermal stability
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Lipase is one kind of enzyme that is widely used as a catalyst in industrial and medical. Lipase has a great catalytic ability, but is easily affected by temperature, and is also difficult to separate at the end of the reaction. This causes most of lipase used only in one reaction cycle. Therefore an enzyme immobilization is needed, so that lipase can maintenance it's activity in high temperatures, and can also be separated at the end of the reaction so that it can be re-used. This study aimed to determine the thermal stability and re-used stability of the immobilized lipase in carrageenan. The method used for immobilizing lipase is through entrapment method. The effectiveness of immobilized lipase tested through the hydrolysis reaction of palm oil. The result was found that the immobilized lipase in carrageenan was able to maintain its catalytic activity up to 50 °C and also up to five reaction cycles.
ISSN:1755-1307
1755-1315
DOI:10.1088/1755-1315/292/1/012028