Features of Brain Astrocyte Damage under the Influence of L-Aminoadipic Acid In Vitro and In Vivo

L-Aminoadipic acid (L-AA) is known to have toxic effects on astroglia. The purpose of the work is to characterize the morphological changes in astrocytes i n vitro and in viv o under the influence of L-AA. The effect of L-AA in the concentration range 0.17–1.4 mM on astrocytes was evaluated in prima...

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Bibliographic Details
Published in:Cell and tissue biology 2021, Vol.15 (4), p.347-355
Main Authors: Voronkov, D. N., Lyzhin, A. A., Dikalova, Yu. V., Stavrovskaya, A. V., Khudoerkov, R. M., Khaspekov, L. G.
Format: Article
Language:English
Subjects:
Astrocytes
Biomedical and Life Sciences
Brain injury
Cell Biology
Cerebellum
Cerebral cortex
Glial fibrillary acidic protein
Injection
Life Sciences
Lysosomes
Neostriatum
Neurodegenerative diseases
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Summary:L-Aminoadipic acid (L-AA) is known to have toxic effects on astroglia. The purpose of the work is to characterize the morphological changes in astrocytes i n vitro and in viv o under the influence of L-AA. The effect of L-AA in the concentration range 0.17–1.4 mM on astrocytes was evaluated in primary dissociated cultures of the rat cerebral cortex and cerebellum, as well as upon stereotaxic injection (20 μg) into the striatum of rats. Concentrations of 0.35–1.4 mM L-AA caused a decrease in the expression of acidic glyofibrillar protein (GFAP), damage and death of astrocytes, pyknosis, and activation of lysosomes (increased LAMP2 expression). On the second day after the injection of L-AA into the striatum of rats, an extensive lesion area devoid of GFAP-positive staining was formed. The data obtained showed that the use of L-aminoadipic acid is promising for modeling damage to astroglia in neurodegenerative diseases.
ISSN:1990-519X
1990-5203
DOI:10.1134/S1990519X21040106