Isolation and preservation of microfungi from Indonesia islands

The research was aimed at utilizing more adequate methods of long-term preservation, cryopreservation (freezing at -80 °C). Samples obtained from three islands were used as sources of samples, i.e., Enggano, Riau, and Sumba. Direct isolation and single spore isolation were conducted to isolate the m...

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Bibliographic Details
Published in:IOP conference series. Earth and environmental science 2019-08, Vol.308 (1), p.12079
Main Author: Wulandari, N F
Format: Article
Language:English
Subjects:
Cryopreservation
Culture collections
Dextrose
Freezing
Growth rate
Islands
Potatoes
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Summary:The research was aimed at utilizing more adequate methods of long-term preservation, cryopreservation (freezing at -80 °C). Samples obtained from three islands were used as sources of samples, i.e., Enggano, Riau, and Sumba. Direct isolation and single spore isolation were conducted to isolate the microfungi. All the cultures were grown on Potato Dextrose Agar (PDA). The growth rate of selected microfungi was calculated every day for a week. The growth rate for selected ten microfungi of each island, approximately 0.1-0.7 cm/day. All the cultures are maintained in InaCC (Indonesian Culture Collections), Cibinong, LIPI.
ISSN:1755-1307
1755-1315
DOI:10.1088/1755-1315/308/1/012079