Sugar concentration of Gracilaria sp. following hydrolysis using cellulase and sulphuric acid and several pretreatment methods

In the search for new and renewable energy, Gracilaria sp. was studied as the raw material for bioethanol production. This seaweed is available abundantly in the very long Indonesian coastline. This study investigates the effect of several pretreatment methods on the concentration of sugar produced...

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Bibliographic Details
Published in:IOP conference series. Earth and environmental science 2019-10, Vol.339 (1), p.12052
Main Authors: Ciawi, Y, Rita, W S, S Anggreni, S A P M
Format: Article
Language:English
Subjects:
Acids
Algae
Biofuels
Cellulase
Ethanol
Gas chromatography
Gracilaria
Hydrolysis
Pretreatment
Reagents
Renewable energy
Seaweeds
Spectrophotometry
Sugar
Sulfuric acid
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Summary:In the search for new and renewable energy, Gracilaria sp. was studied as the raw material for bioethanol production. This seaweed is available abundantly in the very long Indonesian coastline. This study investigates the effect of several pretreatment methods on the concentration of sugar produced from Gracilaria sp. when hydrolyzed using cellulase or sulphuric acid. Reducing sugar was measured by UV-Vis spectrophotometry using Nelson-Somyogi reagent and the ethanol concentration was measured by using gas chromatography. Cellulase and sulphuric acid (H2SO4) were used in the hydrolysis. Cellulase concentration used was 200, 400, 600 and 800 units/ml, whereas the concentration of sulphuric acid used was 1%, 3%%, 5%, and 7%. The highest concentration of reducing sugar was produced by hydrolysis using H2SO4 1%.
ISSN:1755-1307
1755-1315
DOI:10.1088/1755-1315/339/1/012052