DNA barcoding of snakeskin gourami Trichogaster pectoralis and blue bourami Trichogaster trichopterus based on cythocrome c oxidase subunit I (COI) gene

• Published in: IOP conference series. Earth and environmental science 2019-11, Vol.348 (1), p.12031
• Main Authors: Syaifudin, M, Jubaedah, D, Yonarta, D, Hastuti, Z
• Format: Article
• Language: English
• Subjects: COI protein; Cytochrome; Cytochrome-c oxidase; Cytochromes; Deoxyribonucleic acid; DNA; DNA sequencing; Mitochondrial DNA; Nucleotides; Oxidase; Phylogenetics; Phylogeny; Polymerase chain reaction; Similarity; Species; Trichogaster; Trichogaster pectoralis; Trichogaster trichopterus
• ISSN: 1755-1307; 1755-1315
• DOI: 10.1088/1755-1315/348/1/012031

Snakeskin gourami Trichogaster pectoralis and blue gourami Trichogaster trichopterus are the species from the genus of Trichogaster. There are three species of the genus Trichogaster in Indonesia. This research aimed to identify the sequence of cytochrome c oxidase subunit I (COI) gene of mtDNA, and construct the phylogenetic trees among species. The stage of research consisted of DNA isolation, DNA amplification using PCR (Polymerase Chain Reaction) and sequencing COI gene in mtDNA region. Sequencing of the COI gene produced 604 bp for snakeskin gourami and 628bp for blue gourami. The result of BLASTn (Basic Local Aligment Search Tool-nucleotide) analysis showed that snakeskin gourami from Kelekar River had 100% similarity of snakeskin from Indonesia and Philippines and farthest (88%) with snakeskin gourami from France. Blue gourami from Inderalaya had 100% similarity with three spot gourami from Australia, South Africa and India and farthest (88%) with snakeskin gourami from Japan. The phylogenetic tree indicated that snakeskin and blue gourami were clustered in separate branches, where snakeskin gourami had 100% similarity to the same species from Indonesia and the Philippines, and blue gourami had 100% identity to the same species from Japan.