Enzyme-assisted extraction of fatty acid from Caulerpa lentilifera: a preliminary study

Polyunsaturated fatty acids (PUFA) are essential nutrients that play an essential role in the functioning of the brain and cell growth. Mammals, including human, cannot synthesize PUFA. Therefore, the intake of PUFA must be obtained from dietary sources. Marine macroalgae, such as Caulerpa lentilife...

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Published in:IOP conference series. Earth and environmental science 2020-01, Vol.404 (1), p.12040
Main Authors: Rahmawati, S I, Izzati, F, Hapsari, Y, Septiana, E, Rachman, F, Bustanussalam, Simanjuntak, P
Format: Article
Language:English
Subjects:
Algae
Biological activity
Caulerpa
Diet
enzyme-assisted extraction
Enzymes
Essential nutrients
Fatty acids
Lipids
Nutrients
Papain
Polyunsaturated fatty acids
PUFA
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Summary:Polyunsaturated fatty acids (PUFA) are essential nutrients that play an essential role in the functioning of the brain and cell growth. Mammals, including human, cannot synthesize PUFA. Therefore, the intake of PUFA must be obtained from dietary sources. Marine macroalgae, such as Caulerpa lentilifera, are excellent sources for high PUFA. The extraction of PUFA in the pure form is usually carried out by conventional extraction whereas not environmentally friendly. Therefore, using enzymatic-assisted extraction is a great alternative to obtain PUFA from Caulerpa sp. with lower cost and fewer usage of solvents. In this research, we examined the effectivity of enzyme-assisted extraction using papain enzyme because its cheap and easy to find. The optimum extraction method to obtained bioactivity from lipid extract of C. lentiliera by enzyme assisted extraction using papain enzyme was 0.5% concentration of papain, temperature 60°C for 16 hours incubation.
ISSN:1755-1307
1755-1315
DOI:10.1088/1755-1315/404/1/012040