Isolation and toxicity test of Bacillus thuringiensis from Sekayu region soil, South Sumatra on Spodopteralitura

• Published in: IOP conference series. Earth and environmental science 2018-01, Vol.102 (1), p.12066
• Main Authors: Afriani, S R, Pujiastuti, Y, Irsan, C, Damiri, N, Nugraha, S, Sembiring, E R
• Format: Article
• Language: English
• Subjects: Bacillus thuringiensis; Biological control; Catalase; Catalase test; Laboratories; Larvae; Pest control; Pests; Screening; Spodoptera litura; Toxicity; Toxicity testing
• ISSN: 1755-1307; 1755-1315
• DOI: 10.1088/1755-1315/102/1/012066

This study aimed to obtain bacterial isolates B. thuringiensis potential as a biological control against pests Spodoptera litura. The research was conducted at the Laboratory of Pest and Disease Department, Agricultural Faculty of Sriwijaya University, Campus Inderalaya Ogan Ilir, South Sumatera, from March to June 2017. The study was conducted with survey method and laboratory trial. The results showed that of the 50 soil samples from three villages selected through morphological observation, reaction staining, KOH test, catalase test, producing 13 bacterial isolates. Screening of the 13th toxicity of the isolates suspected B. thuringiensis against S. litura larvae was investigated. Based on the toxicity screening test the following facts were obtained: five isolates ie KJ2M2, KJ3E1, KJ3JB1, KJ3D3 and KJ3D5 were lower toxicity than Dipel, two isolates ie KJ3K4 and KJ3D3 which had the same toxicity to Dipel. Five isolates the KJ3E3, KJ3BW5, KJ3JB5, KJ3D1 and LC2, LC3 known to have effectiveness until the seventh day reached 40%. There was one isolate that is KJ3BW5 which was more effective compared to Dipel as comparison.