Analysis on the Spatial Difference of Bacterial Community Structure in Micro-pressure Air-lift Loop Reactor

In order to reveal the spatial difference of the bacterial community structure in the Micro-pressure Air-lift Loop Reactor, the activated sludge bacterial at five different representative sites in the reactor were studied by denaturing gradient gel electrophoresis (DGGE). The results of DGGE showed...

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Bibliographic Details
Published in:IOP conference series. Earth and environmental science 2018-02, Vol.113 (1), p.12053
Main Authors: Wan, L G, Lin, Q, Bian, D J, Ren, Q K, Xiao, Y B, Lu, W X
Format: Article
Language:English
Subjects:
Activated sludge
Bacteria
Biodegradation
Biodiversity
Community structure
Dissolved oxygen
Electrophoresis
Environmental conditions
Environmental degradation
Flora
Gel electrophoresis
Microorganisms
Pollutant removal
Pollutants
Reactors
Sewage treatment
Similarity
Spatial analysis
Substrates
Synergistic effect
Wastewater treatment
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Summary:In order to reveal the spatial difference of the bacterial community structure in the Micro-pressure Air-lift Loop Reactor, the activated sludge bacterial at five different representative sites in the reactor were studied by denaturing gradient gel electrophoresis (DGGE). The results of DGGE showed that the difference of environmental conditions (such as substrate concentration, dissolved oxygen and PH, etc.) resulted in different diversity and similarity of microbial flora in different spatial locations. The Shannon-Wiener diversity index of the total bacterial samples from five sludge samples varied from 0.92 to 1.28, the biodiversity index was the smallest at point 5, and the biodiversity index was the highest at point 2. The similarity of the flora between the point 2, 3 and 4 was 80% or more, respectively. The similarity of the flora between the point 5 and the other samples was below 70%, and the similarity of point 2 was only 59.2%. Due to the different contribution of different strains to the removal of pollutants, it can give full play to the synergistic effect of bacterial degradation of pollutants, and further improve the efficiency of sewage treatment.
ISSN:1755-1307
1755-1315
DOI:10.1088/1755-1315/113/1/012053