Application of RP-HPLC Technique for development of Analytical method for Validation of Metformin hydrochloride from Bulk drug and Dosage form

A Novel Reverse Phase Liquid Chromatographic Method was developed and validated for estimation of metformin hydrochloride in its dosage form i.e. tablets. The reverse phase HPLC analysis was carried out on isocratic system. The column was Hypersil BDS C18 (150mm x 4.6mm, 5µm) with ambient temperatur...

Full description

Saved in:
Bibliographic Details
Published in:Asian Journal of Research in Chemistry 2021-08, Vol.14 (4), p.265-268
Main Authors: Rele, Rajan V, Patil, Sandip P
Format: Article
Language:English
Subjects:
Accuracy
Chromatography
Drug dosages
Liquid chromatography
Metformin
Pharmaceuticals
Quality control
Reproducibility
Retention
Retention time
Spectrophotometry
Standard deviation
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:A Novel Reverse Phase Liquid Chromatographic Method was developed and validated for estimation of metformin hydrochloride in its dosage form i.e. tablets. The reverse phase HPLC analysis was carried out on isocratic system. The column was Hypersil BDS C18 (150mm x 4.6mm, 5µm) with ambient temperature. The mobile phase consisted of buffer: methanol in proportion 90:10 % (v/v). The flow rate was maintained at 1.0ml/ min. The detection was carried out at wavelength 230nm. The method was validated as per ICH guidelines for system suitability, linearity, accuracy and precision. The linear ranges were 50-150µg/ml for metformin hydrochloride, The accuracy and precision were found to be well within the acceptable limit. The method was successfully applied for determination Metformin hydrochloride in dosage form with good recoveries.
ISSN:0974-4169
0974-4150
DOI:10.52711/0974-4150.2021.00045