Phytochemical Study, HPLC Chromatographic Analysis and Antioxidant Activity of Ephedra alata DC. Female Cones Extracts

The aim of this work is a phytochemical study, antioxidant activity and Chromatographic Analysis by HPLC of extracts of the female cones of Ephedra alata DC. Which grows in Oued Souf region (South East of Algeria). According to the results obtained from the estimation of polyphenols and flavonoids c...

Full description

Saved in:
Bibliographic Details
Published in:Asian Journal of Research in Chemistry 2021-08, Vol.14 (4), p.259-264
Main Authors: A, Chouikh, Z, Houba, H, Himeur, F, Alia, E, Adjal
Format: Article
Language:English
Subjects:
Acids
Antioxidants
Ascorbic acid
Calibration
Chlorogenic acid
Chromatography
Cones
Ethanol
Flavonoids
Free radicals
Gallic acid
Herbal medicine
High performance liquid chromatography
Laboratories
Liquid chromatography
Phytochemicals
Polyphenols
Potassium
Reagents
Scavenging
Solvents
Vanillin
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
cited_by cdi_FETCH-LOGICAL-c1266-dd9618fac74dff3b53431b43fb8fd4883848c5652201bfb866dfed771ee7acaf3
cites cdi_FETCH-LOGICAL-c1266-dd9618fac74dff3b53431b43fb8fd4883848c5652201bfb866dfed771ee7acaf3
container_end_page 264
container_issue 4
container_start_page 259
container_title Asian Journal of Research in Chemistry
container_volume 14
creator A, Chouikh
Z, Houba
H, Himeur
F, Alia
E, Adjal
description The aim of this work is a phytochemical study, antioxidant activity and Chromatographic Analysis by HPLC of extracts of the female cones of Ephedra alata DC. Which grows in Oued Souf region (South East of Algeria). According to the results obtained from the estimation of polyphenols and flavonoids content, the methanolic extract has the best content of polyphenols (158.34±2.71mg GAE/g Extract), and the best values of flavonoids (88.50±1.12mg QE/g Extract). The chromatographic analysis by HPLC has identified eight phenolic compounds, which are Gallic Acid, Chlorogenic acid, Vanillic Acid, Vanillin, p-Coumaric acid, Rutin, Naringenin and Quercitin. The antioxidant activity was evaluated by three tests: DPPH• free-radical scavenging, Hemolysis and Reducing power, the results of test scavenging the free-radical DPPH• show the tannins extract had the best scavenging activity capacity than the other extracts (IC50: 14.94±1.34μg/mL), But, in the hemolysis test, all the extracts were proximity except for the aqueous extract that was shown protected of the erythrocytes (50±0.5% of hemolysis percentage). Finally, in the reducing power assay, its results showed that the tannins extract has a best reducing power 27.16±0.25μg/mL in Abs700= 0.5 compared to other extracts.
doi_str_mv 10.52711/0974-4150.2021.00044
format article
fullrecord <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_journals_2559742336</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2559742336</sourcerecordid><originalsourceid>FETCH-LOGICAL-c1266-dd9618fac74dff3b53431b43fb8fd4883848c5652201bfb866dfed771ee7acaf3</originalsourceid><addsrcrecordid>eNo9kF1rwkAQRUNpoWL9CYWFvla7X9kkj5JqLQgV2j4vk_1oItG1u6uYf9-oxae5czkMzEmSR4InKc0IecFFxsecpHhCMSUTjDHnN8ngWt9esyjuk1EI6x6hheCUZYPksKq76FRtNo2CFn3Gve6e0WK1LFFZe7eB6H487OpGoekW2i40AcFW90ts3LHRsI1oqmJzaGKHnEWzXW20BwQtRECv5QTNzQZag0q3NQHNjtGDiuEhubPQBjP6n8Pkez77Khfj5cfbezldjhWhQoy1LgTJLaiMa2tZlTLOSMWZrXKreZ6znOcqFSmlmFR9KYS2RmcZMSYDBZYNk6fL3Z13v3sToly7ve8fCZKmaa-FMiZ6Kr1QyrsQvLFy55sN-E4SLM-W5UmhPOmUJ8vybJn9Abz6b9A</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2559742336</pqid></control><display><type>article</type><title>Phytochemical Study, HPLC Chromatographic Analysis and Antioxidant Activity of Ephedra alata DC. Female Cones Extracts</title><source>Alma/SFX Local Collection</source><creator>A, Chouikh ; Z, Houba ; H, Himeur ; F, Alia ; E, Adjal</creator><creatorcontrib>A, Chouikh ; Z, Houba ; H, Himeur ; F, Alia ; E, Adjal</creatorcontrib><description>The aim of this work is a phytochemical study, antioxidant activity and Chromatographic Analysis by HPLC of extracts of the female cones of Ephedra alata DC. Which grows in Oued Souf region (South East of Algeria). According to the results obtained from the estimation of polyphenols and flavonoids content, the methanolic extract has the best content of polyphenols (158.34±2.71mg GAE/g Extract), and the best values of flavonoids (88.50±1.12mg QE/g Extract). The chromatographic analysis by HPLC has identified eight phenolic compounds, which are Gallic Acid, Chlorogenic acid, Vanillic Acid, Vanillin, p-Coumaric acid, Rutin, Naringenin and Quercitin. The antioxidant activity was evaluated by three tests: DPPH• free-radical scavenging, Hemolysis and Reducing power, the results of test scavenging the free-radical DPPH• show the tannins extract had the best scavenging activity capacity than the other extracts (IC50: 14.94±1.34μg/mL), But, in the hemolysis test, all the extracts were proximity except for the aqueous extract that was shown protected of the erythrocytes (50±0.5% of hemolysis percentage). Finally, in the reducing power assay, its results showed that the tannins extract has a best reducing power 27.16±0.25μg/mL in Abs700= 0.5 compared to other extracts.</description><identifier>ISSN: 0974-4169</identifier><identifier>EISSN: 0974-4150</identifier><identifier>DOI: 10.52711/0974-4150.2021.00044</identifier><language>eng</language><publisher>Raipur: A&amp;V Publications</publisher><subject>Acids ; Antioxidants ; Ascorbic acid ; Calibration ; Chlorogenic acid ; Chromatography ; Cones ; Ethanol ; Flavonoids ; Free radicals ; Gallic acid ; Herbal medicine ; High performance liquid chromatography ; Laboratories ; Liquid chromatography ; Phytochemicals ; Polyphenols ; Potassium ; Reagents ; Scavenging ; Solvents ; Vanillin</subject><ispartof>Asian Journal of Research in Chemistry, 2021-08, Vol.14 (4), p.259-264</ispartof><rights>Copyright A&amp;V Publications Jul/Aug 2021</rights><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c1266-dd9618fac74dff3b53431b43fb8fd4883848c5652201bfb866dfed771ee7acaf3</citedby><cites>FETCH-LOGICAL-c1266-dd9618fac74dff3b53431b43fb8fd4883848c5652201bfb866dfed771ee7acaf3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids></links><search><creatorcontrib>A, Chouikh</creatorcontrib><creatorcontrib>Z, Houba</creatorcontrib><creatorcontrib>H, Himeur</creatorcontrib><creatorcontrib>F, Alia</creatorcontrib><creatorcontrib>E, Adjal</creatorcontrib><title>Phytochemical Study, HPLC Chromatographic Analysis and Antioxidant Activity of Ephedra alata DC. Female Cones Extracts</title><title>Asian Journal of Research in Chemistry</title><description>The aim of this work is a phytochemical study, antioxidant activity and Chromatographic Analysis by HPLC of extracts of the female cones of Ephedra alata DC. Which grows in Oued Souf region (South East of Algeria). According to the results obtained from the estimation of polyphenols and flavonoids content, the methanolic extract has the best content of polyphenols (158.34±2.71mg GAE/g Extract), and the best values of flavonoids (88.50±1.12mg QE/g Extract). The chromatographic analysis by HPLC has identified eight phenolic compounds, which are Gallic Acid, Chlorogenic acid, Vanillic Acid, Vanillin, p-Coumaric acid, Rutin, Naringenin and Quercitin. The antioxidant activity was evaluated by three tests: DPPH• free-radical scavenging, Hemolysis and Reducing power, the results of test scavenging the free-radical DPPH• show the tannins extract had the best scavenging activity capacity than the other extracts (IC50: 14.94±1.34μg/mL), But, in the hemolysis test, all the extracts were proximity except for the aqueous extract that was shown protected of the erythrocytes (50±0.5% of hemolysis percentage). Finally, in the reducing power assay, its results showed that the tannins extract has a best reducing power 27.16±0.25μg/mL in Abs700= 0.5 compared to other extracts.</description><subject>Acids</subject><subject>Antioxidants</subject><subject>Ascorbic acid</subject><subject>Calibration</subject><subject>Chlorogenic acid</subject><subject>Chromatography</subject><subject>Cones</subject><subject>Ethanol</subject><subject>Flavonoids</subject><subject>Free radicals</subject><subject>Gallic acid</subject><subject>Herbal medicine</subject><subject>High performance liquid chromatography</subject><subject>Laboratories</subject><subject>Liquid chromatography</subject><subject>Phytochemicals</subject><subject>Polyphenols</subject><subject>Potassium</subject><subject>Reagents</subject><subject>Scavenging</subject><subject>Solvents</subject><subject>Vanillin</subject><issn>0974-4169</issn><issn>0974-4150</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><recordid>eNo9kF1rwkAQRUNpoWL9CYWFvla7X9kkj5JqLQgV2j4vk_1oItG1u6uYf9-oxae5czkMzEmSR4InKc0IecFFxsecpHhCMSUTjDHnN8ngWt9esyjuk1EI6x6hheCUZYPksKq76FRtNo2CFn3Gve6e0WK1LFFZe7eB6H487OpGoekW2i40AcFW90ts3LHRsI1oqmJzaGKHnEWzXW20BwQtRECv5QTNzQZag0q3NQHNjtGDiuEhubPQBjP6n8Pkez77Khfj5cfbezldjhWhQoy1LgTJLaiMa2tZlTLOSMWZrXKreZ6znOcqFSmlmFR9KYS2RmcZMSYDBZYNk6fL3Z13v3sToly7ve8fCZKmaa-FMiZ6Kr1QyrsQvLFy55sN-E4SLM-W5UmhPOmUJ8vybJn9Abz6b9A</recordid><startdate>20210803</startdate><enddate>20210803</enddate><creator>A, Chouikh</creator><creator>Z, Houba</creator><creator>H, Himeur</creator><creator>F, Alia</creator><creator>E, Adjal</creator><general>A&amp;V Publications</general><scope>AAYXX</scope><scope>CITATION</scope><scope>04Q</scope><scope>04W</scope><scope>8FE</scope><scope>8FG</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>BKSAR</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>HCIFZ</scope><scope>KB.</scope><scope>PCBAR</scope><scope>PDBOC</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope></search><sort><creationdate>20210803</creationdate><title>Phytochemical Study, HPLC Chromatographic Analysis and Antioxidant Activity of Ephedra alata DC. Female Cones Extracts</title><author>A, Chouikh ; Z, Houba ; H, Himeur ; F, Alia ; E, Adjal</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c1266-dd9618fac74dff3b53431b43fb8fd4883848c5652201bfb866dfed771ee7acaf3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Acids</topic><topic>Antioxidants</topic><topic>Ascorbic acid</topic><topic>Calibration</topic><topic>Chlorogenic acid</topic><topic>Chromatography</topic><topic>Cones</topic><topic>Ethanol</topic><topic>Flavonoids</topic><topic>Free radicals</topic><topic>Gallic acid</topic><topic>Herbal medicine</topic><topic>High performance liquid chromatography</topic><topic>Laboratories</topic><topic>Liquid chromatography</topic><topic>Phytochemicals</topic><topic>Polyphenols</topic><topic>Potassium</topic><topic>Reagents</topic><topic>Scavenging</topic><topic>Solvents</topic><topic>Vanillin</topic><toplevel>online_resources</toplevel><creatorcontrib>A, Chouikh</creatorcontrib><creatorcontrib>Z, Houba</creatorcontrib><creatorcontrib>H, Himeur</creatorcontrib><creatorcontrib>F, Alia</creatorcontrib><creatorcontrib>E, Adjal</creatorcontrib><collection>CrossRef</collection><collection>India Database</collection><collection>India Database: Science &amp; Technology</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>Materials Science &amp; Engineering Collection</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central</collection><collection>AUTh Library subscriptions: ProQuest Central</collection><collection>Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Earth, Atmospheric &amp; Aquatic Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central</collection><collection>SciTech Premium Collection</collection><collection>Materials Science Database</collection><collection>Earth, Atmospheric &amp; Aquatic Science Database</collection><collection>Materials Science Collection</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><jtitle>Asian Journal of Research in Chemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>A, Chouikh</au><au>Z, Houba</au><au>H, Himeur</au><au>F, Alia</au><au>E, Adjal</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Phytochemical Study, HPLC Chromatographic Analysis and Antioxidant Activity of Ephedra alata DC. Female Cones Extracts</atitle><jtitle>Asian Journal of Research in Chemistry</jtitle><date>2021-08-03</date><risdate>2021</risdate><volume>14</volume><issue>4</issue><spage>259</spage><epage>264</epage><pages>259-264</pages><issn>0974-4169</issn><eissn>0974-4150</eissn><abstract>The aim of this work is a phytochemical study, antioxidant activity and Chromatographic Analysis by HPLC of extracts of the female cones of Ephedra alata DC. Which grows in Oued Souf region (South East of Algeria). According to the results obtained from the estimation of polyphenols and flavonoids content, the methanolic extract has the best content of polyphenols (158.34±2.71mg GAE/g Extract), and the best values of flavonoids (88.50±1.12mg QE/g Extract). The chromatographic analysis by HPLC has identified eight phenolic compounds, which are Gallic Acid, Chlorogenic acid, Vanillic Acid, Vanillin, p-Coumaric acid, Rutin, Naringenin and Quercitin. The antioxidant activity was evaluated by three tests: DPPH• free-radical scavenging, Hemolysis and Reducing power, the results of test scavenging the free-radical DPPH• show the tannins extract had the best scavenging activity capacity than the other extracts (IC50: 14.94±1.34μg/mL), But, in the hemolysis test, all the extracts were proximity except for the aqueous extract that was shown protected of the erythrocytes (50±0.5% of hemolysis percentage). Finally, in the reducing power assay, its results showed that the tannins extract has a best reducing power 27.16±0.25μg/mL in Abs700= 0.5 compared to other extracts.</abstract><cop>Raipur</cop><pub>A&amp;V Publications</pub><doi>10.52711/0974-4150.2021.00044</doi><tpages>6</tpages></addata></record>
fulltext fulltext
identifier ISSN: 0974-4169
ispartof Asian Journal of Research in Chemistry, 2021-08, Vol.14 (4), p.259-264
issn 0974-4169
0974-4150
language eng
recordid cdi_proquest_journals_2559742336
source Alma/SFX Local Collection
subjects Acids
Antioxidants
Ascorbic acid
Calibration
Chlorogenic acid
Chromatography
Cones
Ethanol
Flavonoids
Free radicals
Gallic acid
Herbal medicine
High performance liquid chromatography
Laboratories
Liquid chromatography
Phytochemicals
Polyphenols
Potassium
Reagents
Scavenging
Solvents
Vanillin
title Phytochemical Study, HPLC Chromatographic Analysis and Antioxidant Activity of Ephedra alata DC. Female Cones Extracts
url http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-03T23%3A42%3A47IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Phytochemical%20Study,%20HPLC%20Chromatographic%20Analysis%20and%20Antioxidant%20Activity%20of%20Ephedra%20alata%20DC.%20Female%20Cones%20Extracts&rft.jtitle=Asian%20Journal%20of%20Research%20in%20Chemistry&rft.au=A,%20Chouikh&rft.date=2021-08-03&rft.volume=14&rft.issue=4&rft.spage=259&rft.epage=264&rft.pages=259-264&rft.issn=0974-4169&rft.eissn=0974-4150&rft_id=info:doi/10.52711/0974-4150.2021.00044&rft_dat=%3Cproquest_cross%3E2559742336%3C/proquest_cross%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c1266-dd9618fac74dff3b53431b43fb8fd4883848c5652201bfb866dfed771ee7acaf3%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=2559742336&rft_id=info:pmid/&rfr_iscdi=true