Rapid and reliable QuEChERS-based LC-MS/MS method for determination of acrylamide in potato chips and roasted coffee

• Published in: IOP conference series. Earth and environmental science 2017-09, Vol.85 (1), p.12010
• Main Authors: Stefanović, S, Đorđevic, V, Jelušić, V
• Format: Article
• Language: English
• Subjects: Acetonitrile; Acrylamide; Coffee; Food industry; Potatoes; Selectivity; Sodium chloride; Vegetables
• ISSN: 1755-1307; 1755-1315
• DOI: 10.1088/1755-1315/85/1/012010

The aim of this paper is to verify the performance characteristics and fitness for purpose of rapid and simple QuEChERS-based LC-MS/MS method for determination of acrylamide in potato chips and coffee. LC-MS/MS is by far the most suitable analytical technique for acrylamide measurements given its inherent sensitivity and selectivity, as well as capability of analyzing underivatized molecule. Acrylamide in roasted coffee and potato chips wasextracted with water:acetonitrile mixture using NaCl and MgSO4. Cleanup was carried out with MgSO4 and PSA. Obtained results were satisfactory. Recoveries were in the range of 85-112%, interlaboratory reproducibility (Cv) was 5.8-7.6% and linearity (R2) was in the range of 0.995-0.999. LoQ was 35 μg kg−1 for coffee and 20 μg kg−1 for potato chips. Performance characteristic of the method are compliant with criteria for analytical methods validation. Presented method for quantitative determination of acrylamide in roasted coffee and potato chips is fit for purposes of self-control in food industry as well as regulatory controls carried out by the governmental agencies.