The effect of SiNPs on DNA methylation of genome in mouse spermatocytes

• Published in: Environmental science and pollution research international 2021-08, Vol.28 (32), p.43684-43697
• Main Authors: Sang, Yujian, Liu, Jianhui, Li, Xiangyang, Zhou, Guiqing, Zhang, Yue, Gao, Leqiang, Zhao, Yanzhi, Zhou, Xianqing
• Format: Article
• Language: English
• Subjects: Apoptosis; Aquatic Pollution; Atmospheric Protection/Air Quality Control/Air Pollution; Deoxyribonucleic acid; DNA; DNA methylation; Earth and Environmental Science; Ecotoxicology; Environment; Environmental Chemistry; Environmental Health; Environmental science; Epigenetics; Gametocytes; Gene expression; Genes; Genomes; Males; Mitochondria; Nanoparticles; Particulate matter; Research Article; Silica; Silicon dioxide; Spermatocytes; Spermatogenesis; Toxicity; Transcription; Waste Water Technology; Water Management; Water Pollution Control
• ISSN: 0944-1344; 1614-7499
• DOI: 10.1007/s11356-021-13459-8

Silica nanoparticles (SiNPs), which are the main inorganic components of atmospheric particulate matter, have been proved to have certain male reproductive toxicity in previous studies. Spermatogenesis involves complex epigenetic regulation, but it is still unclear if SiNPs exposure will interfere with the DNA methylation patterns in mouse spermatocytes. The present study was designed to investigate the effects of SiNPs on DNA methylation in the mouse spermatocyte GC-2spd(ts). GC-2 cells were treated with 0 and 20 μg/mL SiNPs for 24 h. MeDIP-seq assay was then performed to analyze the differentially methylated genes related to spermatogenesis. The results showed that SiNPs induced extensive methylation changes in the genome of GC-2 cells, and 24a total of 428 hyper-methylated genes and 398 hypo-methylated genes were identified. Gene Ontology and pathway analysis showed that differential DNA methylation induced by SiNPs was probably involved with abnormal transcription and translation, mitochondrial damage, and cell apoptosis. Results from qRT-PCR verification showed that the expression of spermatogenesis-related genes Akap1 , Crem , Spz1 , and Tex11 were dysregulated by SiNPs exposure, which was consistent with the MeDIP-seq assay. In general, this study suggested that SiNPs caused genome-wide DNA methylation changes in GC-2 cells, providing valuable reference for the future epigenetic studies in SiNPs-induced male reproductive toxicity.