A clarifying reagent and microplate-based method for the high-throughput analysis of lipase activity in milk

Lipases play an important dual role in the dairy industry. Lipases improve quality but cause deterioration and changes in quality during the processing and storage of dairy products. Therefore, a rapid and sensitive method for determining lipase activity is needed. A new reagent, called a ‘Clarifyin...

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Bibliographic Details
Published in:European food research & technology 2021-09, Vol.247 (9), p.2161-2169
Main Authors: Zhang, Weiqing, Wang, Bo, Ma, Changlu, Pang, Xiaoyang, Lv, Jiaping
Format: Article
Language:English
Subjects:
Agriculture
Analytical Chemistry
Biotechnology
Chemistry
Chemistry and Materials Science
Dairy industry
Dairy products
Food Science
Forestry
Heat treatment
Heat treatments
Lipase
Milk
Milk products
Original Paper
Reagents
Spectrophotometry
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Summary:Lipases play an important dual role in the dairy industry. Lipases improve quality but cause deterioration and changes in quality during the processing and storage of dairy products. Therefore, a rapid and sensitive method for determining lipase activity is needed. A new reagent, called a ‘Clarifying Buffer’, was developed for the clarification of opaque milk and the direct detection of lipase activity in milk using spectrophotometry. The present study successfully used a microplate-based method to analyze lipase activity in milk products. The lipase activities of raw milk samples subjected to different heat treatments were evaluated using the new method. The new method was sensitive to the activity of trace lipase in milk samples treated at 135 °C for 5, 10, and 30 s.
ISSN:1438-2377
1438-2385
DOI:10.1007/s00217-021-03702-4