Effects of Combined Application of Potassium Silicate and Salicylic Acid on the Defense Response of Hydroponically Grown Tomato Plants to Ralstonia solanacearum Infection

• Published in: Sustainability 2021-04, Vol.13 (7), p.3750
• Main Authors: Jiang, Ni-Hao, Zhang, Shi-Han
• Format: Article
• Language: English
• Subjects: Acids; Bacteria; Enzymes; Gene expression; Humidity; Hydroponics; Infections; Invertase; Lipoxygenase; Liquid oxygen; Metabolism; Pathogens; Peroxidase; Phenols; Physiological effects; Plant bacterial diseases; Plant diseases; Plant resistance; Polyphenol oxidase; Potassium; Potassium silicates; Proteins; Ralstonia solanacearum; Salicylic acid; Seeds; Silicon; Sucrose; Sustainability; Synergistic effect; Tomatoes; Virulence; Wilt
• ISSN: 2071-1050; 2071-1050
• DOI: 10.3390/su13073750

Bacterial wilt, caused by soilborne pathogenic bacterium Ralstonia solanacearum, is a serious and widespread disease that affects global tomato production. Both silicon (Si) and salicylic acid (SA) play important roles in enhancing tomato resistance against bacterial wilt, however, their combined effects on the defense responses of infected tomato plants remain unknown. Hence, the combined effects of Si and SA on physiological and biochemical parameters of R. solanacearum-infected tomato plants were investigated. The combination treatment of Si and SA significantly decreased disease incidences, lipoxygenase (LOX) activity and ethylene (ET) production. The combined treatments were more prominent in improving the morphological traits of root systems, such as root length, root surface area, average root diameter and root volume. The activities of polyphenol oxidase (PPO) and peroxidase (POD) and the concentrations of total soluble phenolics (TSPs) and lignin-thioglycolic acid (LTGA) derivatives were significantly increased in the plants with combined treatments. Si in combination with SA could significantly enhance neutral invertase (NI) and acid invertases (AI) activities in the leaves of tomato plants at 3 days post-infection (dpi) compared with application of Si alone. Three defense-related genes, PAL, POD and pathogenesis-related protein 1 (PR1), were significantly induced in Si+SA treatment at 7 dpi when compared with individual application of Si or SA. The expression level of salicylic acid-binding protein 2 (SABP2) was significantly higher for combination treatment when compared with treatment of Si or SA alone. The possible mechanisms involved in the synergistic effects of Si and SA on the control of tomato bacterial wilt were proposed. This study indicates that under hypertonic conditions, the combined application of 2.0 mM potassium silicate (K2SiO3) and 0.5 mM SA had a synergistic effect on the control of tomato bacterial wilt.