Recent Developments and Challenges for the Industrial Implementation of Polyphosphate Kinases

Polyphosphate kinases (PPKs) have emerged as valuable candidates to address the unmet need for scalable recycling of the common enzyme cofactor adenosine‐5’‐triphosphate (ATP) because they use cheap, freely available and stable polyphosphate (polyP) salts as the phosphate donor. The aim of this revi...

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Bibliographic Details
Published in:ChemCatChem 2021-08, Vol.13 (16), p.3565-3580
Main Authors: Tavanti, Michele, Hosford, Joseph, Lloyd, Richard C., Brown, Murray J. B.
Format: Article
Language:English
Subjects:
Adenosine
ATP
biocatalysis
Biocatalysts
cofactor
Kinases
polyphosphate
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Summary:Polyphosphate kinases (PPKs) have emerged as valuable candidates to address the unmet need for scalable recycling of the common enzyme cofactor adenosine‐5’‐triphosphate (ATP) because they use cheap, freely available and stable polyphosphate (polyP) salts as the phosphate donor. The aim of this review is not only to present recent efforts in the characterisation of PPKs but also to provide an overview of the challenges associated with their implementation in chemical manufacturing. In assessing the current status of polyP‐driven biocatalysts, we identify gaps that need to be filled for successful adoption of these biocatalysts in industry. Guidelines for a wider adoption of PPKs are also provided. Industrial biocatalysis: Polyphosphate kinases (PPKs) have recently emerged as promising candidates for scalable ATP recycling. In addition to presenting biochemical attributes of PPKs, the aim of this Review is to identify areas where more investigation is needed and to provide a set of guidelines to direct endeavours aimed at integrating these enzymes in intensified ATP‐dependent reactions.
ISSN:1867-3880
1867-3899
DOI:10.1002/cctc.202100688