IgA1 Protease as a Vaccine Basis for Prevention of Bacterial Meningitis

• Published in: Russian journal of bioorganic chemistry 2021-07, Vol.47 (4), p.805-814
• Main Authors: Zhigis, L. S., Kotelnikova, O. V., Zinchenko, A. A., Karlinsky, D. M., Prokopenko, Yu. A., Rumsh, L. D.
• Format: Article
• Language: English
• Subjects: Antigens; Antiinfectives and antibacterials; Bacteria; Bacterial infections; Biochemistry; Biomedical and Life Sciences; Biomedicine; Bioorganic Chemistry; Gram-positive bacteria; Immunoglobulins; Life Sciences; Literature reviews; Meningitis; Organic Chemistry; Pathogens; Protease; Proteins; Vaccines; Virulence
• ISSN: 1068-1620; 1608-330X
• DOI: 10.1134/S106816202104021X

The review covers the study of the protective properties of IgA1 protease and the possibility of creating a vaccine preparation for the prevention of bacterial meningitis of various origins on its basis. Bacterial meningitis belongs to the group of socially dangerous diseases and is characterized by a severe course, numerous complications and high mortality. The approaches used at present in world practice to create antimicrobial vaccines are based on a narrow targeting against a specific pathogen. The development of a monocomponent vaccine against a wide range of bacterial pathogens with a common virulence factor is still relevant. IgA1 protease, a protein that is one of the main virulence factors of a number of gram-negative and gram-positive bacteria, can serve as such an antigen. Bacterial IgA1 protease is uniquely specific for immunoglobulins A1 (IgA1), cleaving peptide bonds in the hinge regions of the IgA1 in humans and other higher primates. Bacteria, getting on the mucous membrane, destroy IgA1, which acts as the first barrier to protect the body from infections. Neutralization of IgA1 protease at this stage can become an obstacle to the development of infection, hindering the adhesion of a number of pathogens that produce this protein. The data available in the literature on the mechanism of antibacterial protection are scattered and ambiguous. The review considers the literature data and the results of our own experiments on the protective activity of IgA1 protease. We have shown that the recombinant meningococcal IgA1 protease and some of its fragments protect mice from infection with a live virulent culture not only of meningococci of the main epidemic serogroups (A, B, C, and W135), but also of some of the most common virulent pneumococcal serotypes. The data obtained indicate the possibility of creating a monocomponent vaccine against these and, possibly, other bacterial infections. Currently, significant progress has been made in studying the structure and functions of secreted proteins in the bacteria Neisseria meningitidis and Haemophilus influenzae . In this review we describe protein translocation systems of N. meningitidis , which are related to the secretion of proteins in these bacteria, and also present modern data on the functions of these proteins. Analysis of experimental data on the structure of IgA1 protease of N. meningitidis and the formation of immunity during vaccination is of key importance in the development of prophylact