Experimental Verification on the Hypothesis about the Possibility of Molecular Diagnostics of Local Tumor Spread on the Lewis Lung Carcinoma Model

Expression of tumor-associated protein beta-III tubulin (TUBB3) was evaluated quantitatively by immunofluorescence analysis using flow cytometry in lung tissue of intact animals and on day 11 after the Lewis lung carcinoma transplantation as well in lung metastasis tissue and in visually “normal” su...

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Bibliographic Details
Published in:Moscow University chemistry bulletin 2021-07, Vol.76 (4), p.248-252
Main Authors: Bogush, T. A., Maiak, M. A., Saprykina, N. S., Basharina, A. A., Ryabinina, O. M., Grishanina, A. N., Bogush, E. A., Kamensky, A. A., Kosorukov, V. S.
Format: Article
Language:English
Subjects:
Animals
Chemistry
Chemistry and Materials Science
Chemistry/Food Science
Flow cytometry
Hypotheses
Immunofluorescence
Lungs
Markers
Proteins
Transplantation
Tumors
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Summary:Expression of tumor-associated protein beta-III tubulin (TUBB3) was evaluated quantitatively by immunofluorescence analysis using flow cytometry in lung tissue of intact animals and on day 11 after the Lewis lung carcinoma transplantation as well in lung metastasis tissue and in visually “normal” surrounding tissue. It was shown that the tumor is characterized by a high TUBB3 expression, while also the expression of the marker was detected in both variants of visually normal lung tissue: it was lower than in tumor tissue but significantly higher than in lung tissue of intact animals. The detection of TUBB3 outside the tumor indicates that the tissue appeared to be normal has already involved in malignancy and this supports the hypothesis that tumor-associated protein TUBB3 can be used as a molecular marker of local tumor spread.
ISSN:0027-1314
1935-0260
DOI:10.3103/S0027131421040039