Development of an electrochemical biosensor for determination of dopamine by gold modified poly(thiophene-3-boronic acid)-polyphenol oxidase modified electrode

•Incorporation of PPO enzyme on AuNPs-PT3BA electrode by amperometric method.•The film exhibits the feasibility of detecting trace level of dopamine.•The biosensor exhibit excellent sensing capability with selectivity for dopamine. The amperometric deposition of poly(thiophene-3-boronic acid) (PT3BA...

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Bibliographic Details
Published in:Materials letters 2021-11, Vol.302, p.130387, Article 130387
Main Authors: Sethuraman, Venkatesan, Sridhar, T.M., Sasikumar, Raman
Format: Article
Language:English
Subjects:
Amperometric
Biosensor
Biosensors
Charge transfer
Current carriers
Differential pulse voltammetry
Dopamine
Electrical measurement
Electrodes
Glassy carbon
Gold
Gold nanoparticles
Materials science
Michaelis-Menten
Neurotransmitters
Polyphenol oxidase
Tyrosinase
Voltammetry
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Summary:•Incorporation of PPO enzyme on AuNPs-PT3BA electrode by amperometric method.•The film exhibits the feasibility of detecting trace level of dopamine.•The biosensor exhibit excellent sensing capability with selectivity for dopamine. The amperometric deposition of poly(thiophene-3-boronic acid) (PT3BA) in presence of glutaraldehdye and tyrosinase enzyme (PPO) on Gold nanopartilces (AuNPs) deposited glassy carbon electrode (GCE) forms a newer AuNPs-PT3BA-PPO electrode which was used to detect dopamine, a neurotransmitter. The charge transfer studies were carried out using cyclic voltammetry. Differential Pulse Voltammetry (DPV) technique was used to detect dopamine. Linear range of detection was 5 × 10−8 to 3 × 10−5 M and limit of detection was 2 × 10−8 M. The derived values for Michaelis-Menten constant (Km) and activation energy were 5.33 µM, respectively. AuNPs-PT3BA-PPO is suitability biosensor for the trace determinations of dopamine.
ISSN:0167-577X
1873-4979
DOI:10.1016/j.matlet.2021.130387