pH-responsive Adsorption and Dissociation of Sialic Acid Expressed Protein on Boronic Acid Immobilized Surface

Detection and isolation of sialic acid (SA) over-expressed biomolecules is of continuous interest in cancer-related research. Boronic acid-based SA-recognition chemistry provides a synthetic molecular basis for such techniques. 5-Boronopicolinic acid, a derivative with the reportedly highest SA-bind...

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Bibliographic Details
Published in:Chemistry letters 2021-08, Vol.50 (8), p.1467-1469
Main Authors: Horiguchi, Yukichi, Barthelmes, Kevin, Miyahara, Yuji, Matsumoto, Akira
Format: Article
Language:English
Subjects:
Acids
Biomolecules
Blood circulation
Cancer
Glycoproteins
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Summary:Detection and isolation of sialic acid (SA) over-expressed biomolecules is of continuous interest in cancer-related research. Boronic acid-based SA-recognition chemistry provides a synthetic molecular basis for such techniques. 5-Boronopicolinic acid, a derivative with the reportedly highest SA-binding strength, was immobilized to a gold surface and studied for its interaction with fetuin, a blood-circulating glycoprotein implicated in cancers. A submicron-molar level sensitivity was achievable, while the sample could be readily dissociated by modulating the pH.
ISSN:0366-7022
1348-0715
DOI:10.1246/cl.210229