Immobilization and plasmon-enhanced fluorescence of EGFP on Al nanohole arrays

Enhanced Green Fluorescent Protein has been covalently immobilized on thin perforated Al films with short-order nanohole arrays using (3-Glycidyloxypropyl)trimethoxysilane and native aluminum oxide layer. The nanoholes' diameter varied in range 60-120 nm, allowing to tune the surface plasmon re...

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Bibliographic Details
Published in:Journal of physics. Conference series 2020-03, Vol.1461 (1), p.12016
Main Authors: Bochenkov, V E, Shakhov, A M, Astafiev, A A, Timoshenko, V A
Format: Article
Language:English
Subjects:
Aluminum oxide
Arrays
Fluorescence
Physics
Surface plasmon resonance
Thin films
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Summary:Enhanced Green Fluorescent Protein has been covalently immobilized on thin perforated Al films with short-order nanohole arrays using (3-Glycidyloxypropyl)trimethoxysilane and native aluminum oxide layer. The nanoholes' diameter varied in range 60-120 nm, allowing to tune the surface plasmon resonance frequency. The fluorescence intensity enhancement up to factor 2.75 for the nanohole arrays with the largest overlap between EGFP fluorescence band and surface plasmon resonance.
ISSN:1742-6588
1742-6596
DOI:10.1088/1742-6596/1461/1/012016