An environmentally-friendly perlecan protein expression

• Published in: Journal of physics. Conference series 2020-02, Vol.1469 (1), p.12112
• Main Authors: Njoto, I, Soekanto, A, Ernawati, S, Kalim, H, Handono, K, Soeatmadji, D W, Abdurrachman, A, Fatchiyah, F
• Format: Article
• Language: English
• Subjects: Arthritis; Biomedical materials; Carbohydrates; Cartilage; Diet; Group dynamics; Growth factors; Hyperglycemia; Osteoarthritis; Pathogenesis; Physics; Protein expression; Proteins; Regeneration; Risk
• ISSN: 1742-6588; 1742-6596
• DOI: 10.1088/1742-6596/1469/1/012112

The risk of high sugar level exposure can affect almost all parts of the body, organ systems, tissues, to the cellular level. The exposure to high sugar level caused disturbances in the joints in the form of changes in the microcellular environment. It will cause negative impact to microcellular component of articular cartilage, which increased the risk of joint degradation. Present study was to investigate excess carbohydrate diets on perlecan protein expression and Transforming Growth Factor alpha (TGF-ᾳ) in articular cartilage which still unclear, which perlecan function to chondrocytes articular cartilage was to support regeneration of articular cartilage by increasing the secretion of anabolic factors by chondrocytes, so the progressivity of articular cartilage damage decreased and the risk of osteoarthritis disease can be lowered too. Function of TGF-ᾳ expression was opposite to perlecan protein expression. Methods: six groups of eighteen adult's male rats were divided into: control (K0), lower diet 1 (A), lower diet 2 (B), middle diet (C), over diet 1 (D), over diet 2 (E). Control group were not performed Anterior Cruciate Ligament Transection (ACLT), but others group were performed ACLT to develop osteoarthritis condition for two months' maintenance. Perlecan protein expression, and expression of TGF-ᾳ were analyzed using Western Blotting method. Perlecan protein expression was fluctuate stable at group (a) < (b) compare to control group (K0), but decreasing at group (e) < (d) < (c) compare to control group, which opposite to increasing carbohydrate intake dose. TGF-ᾳ as catabolic factor was fluctuate increasing at group D > group C > E compare to control group, but slightly stable at group A and B. Interaction of perlecan and TGF-ᾳ as one of others factor which determine cartilage health under hyperglycemia condition. Results and discussion: This aim as early research as a based to next research for accomplish of pathogenesis of osteoarthritis caused by hyperglycemia. Present study concludes that hyperglycemia was influence to cartilage health due to changing of perlecan protein expression and TGF-ᾳ expression.