A ratiometric near-infrared fluorescent probe with a large emission peak shift for sensing and imaging hypochlorous acid

• Published in: Sensors and actuators. B, Chemical Chemical, 2021-09, Vol.343, p.130063, Article 130063
• Main Authors: He, Meng, Ye, Miantai, Wang, Zhengguo, Liu, Pan, Li, Huiyue, Lu, Chunfeng, Wang, Yanying, Liang, Tao, Li, Haiyan, Li, Chunya
• Format: Article
• Language: English
• Subjects: Biomarkers; Diabetic wound healing; Emission; Fluorescent imaging; Fluorescent indicators; Hyperglycemia; Hypochlorous acid; Inflammatory response; Metabolic disorders; Monitoring; Near-infrared; Raiometric; Selectivity; Synthesis; Wound healing
• ISSN: 0925-4005; 1873-3077
• DOI: 10.1016/j.snb.2021.130063

[Display omitted] •A ratiometric near-infrared fluorescent probe (CX-MTC) was developed for HOCl.•CX-MTC with a large peak-to-peak separation of 140 nm can avoid the crosstalk of excitation and emission signals.•CX-MTC exhibits excellent selectivity, sensitivity, low cytotoxicity, and photostability.•Imaging HOCl in PC 12 cells, living zebrafish and wound of diabetic mice was achieved with CX-MTC. Diabetes, a kind of metabolic disease with characteristics of chronic hyperglycemia, can trigger a series of complications. Among them, wound healing prolonging is one of the manifestations of complications. Hypochlorous acid (HOCl) is a ubiquitous reactive oxygen species (ROS) that plays a vital role in inflammatory response and has been considered as a biomarker for monitoring a wound healing process. Herein, a ratiometric near-infrared (NIR) fluorescent probe, CX-MTC, was synthesized and applied for monitoring HOCl. CX-MTC possesses a simple structure and can be easy synthesized. The probe shows itself emission at 525 nm, a new emission at 665 nm is observed after the reaction with HOCl with a large emission shift of 140 nm. CX-MTC provides excellent selectivity and high sensitivity towards HOCl with 63-fold fluorescence intensity ratio enhancement. The probe can be used for monitoring HOCl in cells. Moreover, CX-MTC was successfully used for imaging HOCl in OGD/R cell model. It is also the first fluorescent probe for HOCl detection in diabetic wound healing process, which will be a potentially useful strategy for clinical assay.