Reproductive and metabolic adaptation to multistressor training in women

• Published in: American journal of physiology: endocrinology and metabolism 2021-08, Vol.321 (2), p.E281-E291
• Main Authors: Gifford, Robert M., O’Leary, Thomas J., Wardle, Sophie L., Double, Rebecca L., Homer, Natalie Z. M., Howie, A. Forbes, Greeves, Julie P., Anderson, Richard A., Woods, David R., Reynolds, Rebecca M.
• Format: Article
• Language: English
• Subjects: 17β-Estradiol; Absorptiometry; Adaptation; Adipose tissue; Body composition; Body fat; Dual energy X-ray absorptiometry; Fat-free body mass; Follicle-stimulating hormone; Gonadotropin-releasing hormone; Hypothalamic-pituitary-gonadal axis; Hypothalamus; Inhibin; Insulin; Insulin resistance; Leptin; Luteinizing hormone; Metabolism; Military; Military training; Ovulation; Pituitary; Pituitary (anterior); Progesterone; Sex hormones; Steroid hormones; Training
• ISSN: 0193-1849; 1522-1555
• DOI: 10.1152/ajpendo.00019.2021

We characterized reproductive endocrine adaptation to prolonged arduous multistressor training in women. We identified marked suppression of hypothalamic-pituitary-gonadal (HPG) axis function during training but found no evidence of low energy availability despite high energy requirements. Our findings suggest a complex interplay of psychological and environmental stressors with suppression of the HPG axis via activation of the hypothalamic-pituitary adrenal (HPA) axis. The neuroendocrine impact of nonexercise stressors on the HPG axis during arduous training should be considered. Hypothalamic-pituitary-gonadal (HPG) axis suppression in exercising women can be caused by low energy availability (EA), but the impact of a real-world, multistressor training environment on reproductive and metabolic function is unknown. This study aimed to characterize reproductive and metabolic adaptation in women undertaking basic military training. A prospective cohort study in women undertaking 11-month initial military training ( n = 47) was carried out. Dynamic low-dose 1-h gonadotrophin-releasing hormone (GnRH) tests were completed after 0 and 7 mo of training. Urine progesterone was sampled weekly throughout. Body composition (dual X-ray absorptiometry), fasting insulin resistance (homeostatic modeling assessment 2, HOMA2), leptin, sex steroids, anti-Müllerian hormone (AMH), and inhibin B were measured after 0, 7, and 11 mo with an additional assessment of body composition at 3 mo. Luteinizing hormone (LH) and follicle-stimulating hormone (FSH) responses were suppressed after 7 mo (both P < 0.001). Among noncontraceptive users ( n = 20), 65% had regular (23–35 days) cycles preenrollment, falling to 24% by 7 mo of training. Of women in whom urine progesterone was measured ( n = 24), 87% of cycles showed no evidence of ovulation. There was little change in AMH, LH, and estradiol, although inhibin B and FSH increased ( P < 0.05). Fat mass fluctuated during training but at month 11 was unchanged from baseline. Fat-free mass did not change. Visceral adiposity, HOMA2, and leptin increased (all P < 0.001). HPG axis suppression with anovulation occurred in response to training without evidence of low EA. Increased insulin resistance may have contributed to the observed pituitary and ovarian dysfunction. Our findings are likely to represent an adaptive response of reproductive function to the multistressor nature of military training. NEW & NOTEWORTHY We characterized reproducti