Morpho-physiological characterization coupled with expressional accord of exclusion mechanism in wild and cultivated lentil under aluminum stress

• Published in: Protoplasma 2021-09, Vol.258 (5), p.1029-1045
• Main Authors: Singh, Chandan Kumar, Singh, Dharmendra, Sharma, Shristi, Chandra, Shivani, Taunk, Jyoti, Konjengbam, Noren Singh, Singh, Deepti, Kumar, Arun, Upadhyaya, K. C., Pal, Madan
• Format: Article
• Language: English
• Subjects: Acidic soils; Acids; Aluminum; Aluminum - toxicity; Antioxidants; Biomedical and Life Sciences; Cell Biology; Chromosomes; Citrate synthase; Cultivars; Enzymatic activity; Gene families; Genomes; Genotypes; Humans; Lens Plant - genetics; Life Sciences; Localization; Nucleotide sequence; Original Article; Physiology; Plant Roots; Plant Sciences; Pyruvate carboxylase; Pyruvic acid; Seedlings; Seeds; Soil; Toxicity; Zoology
• ISSN: 0033-183X; 1615-6102
• DOI: 10.1007/s00709-021-01619-z

Aluminum stress deteriorates lentil production under acidic soils. Enhanced insight into Al tolerance traits is needed to improve its productivity. Therefore, Al-resistant (L-4602, PAL-8) and Al-sensitive (BM-4, EC-223229) cultivars along with a resistant wild (ILWL-15) were characterized for morpho-physiological traits viz. seedling root architecture (SRA), Al accumulation, and localization via fluorescent and non-fluorescent staining under control and Al-treated conditions. Also, antioxidant activities and organic acid secretion were quantified, and expressions of 10 associated genes were analyzed. Roots of Al-resistant cultivars and wild genotype showed higher root growth, antioxidant enzyme activities, and organic acid secretion than Al-sensitive ones. Among these traits, higher organic acid secretion was influenced by enhanced expression of genes, especially—aluminum sensitive-3 (ALS 3), aluminum-activated malate transporter (ALMT), multidrug and toxic compound extrusion (MATE), citrate synthase (CS), and phospho enol pyruvate carboxylase (PEPC)—which helped in reducing Al and callose accumulation. These genes were located on lentil chromosomes via sequence alignment with lentil draft genome. A strong link between morpho-physiological variation and organic acid secretion was noted which reinforced the prominence of exclusion mechanism. It was complemented by enhanced antioxidant activities at seedling stage which mitigated Al stress effects on SRA. Wild outperformed over cultivars indicating its impregnable evolution which can be exploited to better understand tolerance mechanisms. Al-resistant cultivars had significantly higher seed yield than Al-sensitive and national checks on Al-toxic fields, confirming—tolerance is sustained till reproductive stage in lentil. This study elucidated role of gene families in eliminating Al toxicity that will assist breeders to formulate strategies for developing Al-resistant cultivars.