Evaluation of Total Phenolic, Total Flavonoid, and In Vitro Cytotoxic Activity of Syzygium cumini Extract in Cervical Cancer Cell

Side effect of conventional cancer terapy has driven researches to find alternative therapy. People in the province of West Nusa Tenggara, especially the Sasak tribe, perform medical treatments that refer to the traditional Lontar Usada manuscript. One of the plants mentioned in this manuscript was...

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Published in:Journal of physics. Conference series 2020-10, Vol.1665 (1), p.12033
Main Authors: Fiqri, Hairil, Arda, Adzani Gaisani, Adrebi, Khodijah, Proborini, Wahyu Diah, Widyanto, Rahma Micho
Format: Article
Language:English
Subjects:
Alkanes
Alkenes
Aromatic compounds
Cancer
Cervical cancer
cytotoxic
Cytotoxicity
Ethyl acetate
Flavonoids
Functional groups
HeLa
Methanol
Nitro compounds
Nitrogen dioxide
Phenols
Physics
Thin layer chromatography
Toxicity testing
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Summary:Side effect of conventional cancer terapy has driven researches to find alternative therapy. People in the province of West Nusa Tenggara, especially the Sasak tribe, perform medical treatments that refer to the traditional Lontar Usada manuscript. One of the plants mentioned in this manuscript was jamblang plant (Syzygium cumini). This study aims to explore and determine the potential of S. cumini leaves extract as an anti-cervical cancer. Extraction was carried out by the Soxhlet method using ethyl acetate, methanol, and water as solvents. The extract obtained was tested for Thin Layer Chromatography (TLC), FTIR test, and cytotoxicity test for the MTT method using HeLa cells. TLC exhibited that the extract contain phenol and flavonoid. FTIR analyzed that the extract had functional groups O-H phenols, C-H alkanes, C=C alkenes, C=0 ketone, C=C aromatic ring, NO2 nitro compound, and C=C alkenes. Total phenolic content and total flavonoid content of ethyl acetate, methanolic and water extract were 443.80 + 0.33; 305.80 + 0.28; and 45.80 + 0.11 mg GAE / g, and 74 + 0.12; 70 + 0.28; and 34 + 0.21 mg QE / g. Ethyl acetate extract showed highest cytotoxicity with IC50 value 330.50 + 1.59, followed by methanol extract 378.35 + 2.84 and water extract 3608.84 + 0.85.
ISSN:1742-6588
1742-6596
DOI:10.1088/1742-6596/1665/1/012033