Histology of some major immune system organs in Lake Van fish Alburnus tarichi (Güldenstädt, 1814) (Cyprinidae)

• Published in: Anatomia, histologia, embryologia histologia, embryologia, 2021-09, Vol.50 (5), p.770-780
• Main Authors: Kaptaner, Burak, Aykut, Handan, Doğan, Emine, Bostancı, Müşerref
• Format: Article
• Language: English
• Subjects: Alburnus tarichi; Anadromous species; Corpuscles; Erythrocytes; fish histology; Histology; Immune system; kidney; Kidneys; Leukocytes; Lymphoid cells; Lymphoid tissue; Macrophages; Paraffin; Red pulp; Spleen; Thymus; Thymus gland
• ISSN: 0340-2096; 1439-0264
• DOI: 10.1111/ahe.12723

The teleostean immune system is variable between and within taxa in terms of morphology. Accordingly, in the current study, the histology of some immune organs (thymus, kidney and spleen) of Lake Van fish (Alburnus tarichi) was investigated. For this purpose, the tissues from eight mature fish were fixed and embedded in paraffin wax, and then the sections were stained with haematoxylin and eosin, Mallory’s triple, periodic acid‐Schiff and Alcian blue (pH of 2.5) for histologic examinations. In the thymus, no clear discrimination of the cortex and medulla was observed. Lymphoid cells within a reticulo‐epithelial network were the predominant cell type in the thymus. The other components in the thymus were macrophages and myoid, mast‐like, plasma‐like, cystic cells, Hassall’s corpuscles, and single or multiple epithelial cystic structures. In the kidney, the head kidney, a major haematopoietic site, consisted of lymphoid and non‐lymphoid zones within a reticular network. Cells exhibiting mitotic figures were also detected in the haematopoietic tissue of the head kidney. Haematopoietic tissue was also found in the trunk kidney dispersed amongst the excretory components. The spleen was composed of red and white pulp. The red pulp comprised abundant erythrocytes, whilst the white pulp contained leucocytes with a reticular network. Ellipsoids were also determined in the white pulp. Melanomacrophage centres were found in all of the examined lymphoid tissues of the fish. These findings, which were reported, herein, for the first time will provide reference knowledge for future studies of this anadromous fish.