The dosage optimization of He-Ne laser energy as a candidate for photodynamic therapy of cancer cells with exogenous photosensitizer variations

• Published in: Journal of physics. Conference series 2017-05, Vol.853 (1), p.12034
• Main Authors: Apsari, R, Maghfiroh, I, Setyawati, H, Winarno, Arifianto, D, Zaidan, A
• Format: Article
• Language: English
• Subjects: Apoptosis; Cancer; Cell death; Chlorophyll; Dosage; Energy; Laser outputs; Lasers; Optimization; Photodynamic therapy; Physics; Toxicity testing
• ISSN: 1742-6588; 1742-6596
• DOI: 10.1088/1742-6596/853/1/012034

The aim of this research was to find out the most effective energy dose of laser energy per unit area irradiated to the subject, used in T47D cancer cell photodynamic therapy. The experiment used three variations of energy doses and two exogenous photosensitizers. The laser used was the He-Ne laser with a wavelength of 632 nm. Laser exposure on the T47D cancer cells was conducted in vitro at a distance of 1 cm with energy doses of 30.4 J/cm2, 18.8 J/cm2, and 30.4 J/cm2. The photosensitizers used in the experiment were chlorophylls isolated from sour-sop leaves and Protoporphyrin IX (PpIX). The concentration of photosensitizers which is not toxic to cells, based on the toxicity test, is 300 ppm for chlorophylls and 350 ppm for PpIX. At the energy dose of 30.4 J/cm2, the percentage of cell death was 96.25% with chlorophyll addition, while with PpIX addition, the percentage was 81.62%. At the same energy dose, cell death in apoptosis was 95.78% with chlorophyll addition and 80.56% with PpIX addition. Based on the result of this research, it was revealed that the energy dose of He-Ne laser output of 30.4 J/cm2 was the most effective energy dose between the energy dose variation that could yield T47D cell death in an apoptosis way.