GC-MS Identification of Anti-inflammatory and Anticancer Metabolites in Edible Milky White Mushroom (Calocybe indica) against Human Breast Cancer (MCF-7) Cells

• Published in: Research journal of pharmacy and technology 2021-08, Vol.14 (8), p.4300-4306
• Main Authors: S., Mohanasundaram, N., Rangarajan, V., Sampath, K., Porkodi, Dass Prakash, M.V., N., Monicka
• Format: Article
• Language: English
• Subjects: Amino acids; Antioxidants; Breast cancer; Cell cycle; Colorectal cancer; Ethanol; Free radicals; Mushrooms; Nonsteroidal anti-inflammatory drugs; Proteins
• ISSN: 0974-3618; 0974-360X; 0974-306X
• DOI: 10.52711/0974-360X.2021.00747

Milky White Mushroom is a species of edible mushroom native to India. The Purpose of this study was to determine the anti-inflammatory and anticancer compounds from Calocybe indica. The ethanolic extract from Calocybe indica were prepared. GC-MS was performed and the major bioactive compounds such as polysaccharides, amino acids, sterols, phytol, and squalene were determined. Calocybe indica was analyzed for their antioxidant activity through In vitro assays such as Free radical activity on DPPH, Hydroxyl radical scavenging assay, Nitric Oxide scavenging assay, Superoxide scavenging assay. Anti-inflammatory effect was estimated by protein denaturation inhibition assay, Proteinase inhibition assay, Cyclooxygenase inhibition assay and Lipoxygenase inhibition assay. Anticancer activity of Calocybe indica extract showed maximum inhibition of 69.11% of growth of human breast cancer cell (MCF 7) at 100μg/ml exposure for about 72 hours. At the end of this study, it was indicated that ethanolic extract of Calocybe indica can be used as an anti- inflammatory and anticancer agent.