Valorization of waste cabbage leaves by postharvest photochemical treatments monitored with a non-destructive fluorescence-based sensor

• Published in: Journal of photochemistry and photobiology. B, Biology Biology, 2021-09, Vol.222, p.112263, Article 112263
• Main Authors: Kowalski, Artur, Agati, Giovanni, Grzegorzewska, Maria, Kosson, Ryszard, Kusznierewicz, Barbara, Chmiel, Tomasz, Bartoszek, Agnieszka, Tuccio, Lorenza, Grifoni, Daniele, Vågen, Ingunn M., Kaniszewski, Stanislaw
• Format: Article
• Language: English
• Subjects: Accumulation; Biosynthesis; Brassica; Brassica oleracea; Brassica oleracea L; Broadband; Chlorophyll fluorescence; Flavonoids; Flavonols; Fluorescence; Fluorescent lamps; Glycosides; High-performance liquid chromatography; Irradiance; Irradiation; Kaempferol; Leaves; Liquid chromatography; Macromolecules; Non-destructive sensors; Optical measuring instruments; Optimization; Photochemicals; Plant extracts; Polyphenols; Postharvest irradiation; Quercetin; Radiation dosage; Solar radiation; Sun; Sunlight; Ultraviolet radiation; Vegetable waste valorisation; Waste management; White light
• ISSN: 1011-1344; 1873-2682
• DOI: 10.1016/j.jphotobiol.2021.112263

The biosynthesis of polyphenolic compounds in cabbage waste, outer green leaves of white head cabbage (Brassica oleracea L. var. capitata subvar. alba), was stimulated by postharvest irradiation with UVB lamps or sunlight. Both treatments boosted the content of kaempferol and quercetin glycosides, especially in the basal leaf zone, as determined by the HPLC analysis of leaf extracts and by a non-destructive optical sensor. The destructive analysis of samples irradiated by the sun for 6 days at the end of October 2015 in Skierniewice (Poland) showed an increase of leaf flavonols by 82% with respect to controls. The treatment by a broadband UVB fluorescent lamp, with irradiance of 0.38 W m−2 in the 290–315 nm range (and 0.59 W m−2 in the UVA region) for 12 h per day at 17 °C along with a white light of about 20 μmol m−2 s−1, produced a flavonols increase of 58% with respect to controls. The kinetics of flavonols accumulation in response to the photochemical treatments was monitored with the FLAV non-destructive index. The initial FLAV rate under the sun was proportional to the daily radiation doses with a better correlation for the sun global irradiance (R2 = 0.973), followed by the UVA (R2 = 0.965) and UVB (R2 = 0.899) irradiance. The sunlight turned out to be more efficient than the UVB lamp in increasing the flavonols level of waste leaves, because of a significant role played by UVA and visible solar radiation in the regulation of the flavonoid accumulation in cabbage. The FLAV index increase induced on the adaxial leaf side was accompanied by a lower but still significant FLAV increase on the unirradiated abaxial side, likely due to a systemic signaling by mean of the long-distance movement of macromolecules. Our present investigation provides useful data for the optimization of postharvest photochemical protocols of cabbage waste valorization. It can represent a novel and alternative tool of vegetable waste management for the recovery of beneficial phytochemicals. [Display omitted] •Sunlight and UVB postharvest irradiation increase flavonols in waste cabbage leaves.•Non-destructive fluorescence sensors control bioactive compounds in cabbage waste.•Sunlight is an efficient and sustainable valorisation tool of cabbage waste leaves.•Accumulation of flavonols in the basal leaf parts is proportional to irradiance.•Signaling from irradiated leaf sides induces flavonols on unirradiated leaf sides.