Detection of single bacteria – causative agents of meningitis using raman microscopy

Early diagnostics of meningitis is a very topical problem as it is a fulminant disease with a high level of mortality. The progress of this disease is, as a rule, accompanied by the appearance of bacteria in the cerebrospinal fluid (CSF) composition. The examination of the CSF is well known to be th...

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Bibliographic Details
Published in:Journal of physics. Conference series 2015-03, Vol.594 (1), p.12029
Main Authors: Baikova, T V, Minaeva, S A, Sundukov, A V, Svistunova, T S, Bagratashvili, V N, Alushin, M V, Gonchukov, S A
Format: Article
Language:English
Subjects:
Bacteria
Cerebrospinal fluid
Line spectra
Meningitis
Microscopy
Optics
Physics
Raman spectra
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Summary:Early diagnostics of meningitis is a very topical problem as it is a fulminant disease with a high level of mortality. The progress of this disease is, as a rule, accompanied by the appearance of bacteria in the cerebrospinal fluid (CSF) composition. The examination of the CSF is well known to be the only reliable approach to the identification of meningitis. However, the traditional biochemical analyses are time consuming and not always reliable, simple, and inexpensive, whereas the optical methods are poorly developed. This work is devoted to the study of Raman spectra of several bacterial cultures which are mainly present during meningitis. Raman microscopy is a prompt and noninvasive technique capable of providing reliable information about molecular-level alterations of biological objects at their minimal quantity and size. It was shown that there are characteristic lines in Raman spectra which can be the reliable markers for determination of bacterial form of meningitis at a level of a single bacterium.
ISSN:1742-6596
1742-6588
1742-6596
DOI:10.1088/1742-6596/594/1/012029