Determination of inorganic elements in blood of mice immunized with Bothrops Snake venom using XRF and NAA

In this work, mice genetically modified [HIII line] were immunized against different Bothrops snake venoms to produce anti-Bothrops serum (antivenom). The Neutron Activation Analysis (NAA) and Energy Dispersive X-Ray Fluorescence (EDXRF) techniques were used to evaluate Ca and Fe concentrations in b...

Full description

Saved in:
Bibliographic Details
Published in:Journal of physics. Conference series 2015-07, Vol.630 (1), p.12005
Main Authors: da Silva, L F F Lopes, Zamboni, C B, Bahovschi, V, Metairon, S, Suzuki, M F, Sant' Anna, O A, Rizzutto, M A
Format: Article
Language:English
Subjects:
Antibodies
Blood
Genetic modification
Immunization
Immunology
Neutron activation analysis
Physics
Snakes
Venom toxins
X-ray fluorescence
Citations: Items that this one cites
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:In this work, mice genetically modified [HIII line] were immunized against different Bothrops snake venoms to produce anti-Bothrops serum (antivenom). The Neutron Activation Analysis (NAA) and Energy Dispersive X-Ray Fluorescence (EDXRF) techniques were used to evaluate Ca and Fe concentrations in blood of these immunized mice in order to establish a potential correlation between both phenotypes: antibody response and blood constituents after Bothrops venom administration. The results were compared with the control group (mice not immunized) and with human being estimative. These data are important for clinical screening of patients submitted to immunological therapy as well as the understanding of the envenoming mechanisms.
ISSN:1742-6588
1742-6596
DOI:10.1088/1742-6596/630/1/012005