Antioxidant Activity of Spiranthes sinensis and Its Protective Effect against UVB-Induced Skin Fibroblast Damage

Spiranthes sinensis (S. sinensis), a species of orchid, is a well-known herb medicine used to treat disorders such as stomachache, diabetes, shingles, and certain inflammatory diseases. Presently, the antioxidant activity of S. sinensis as well as its protective effect on UVB-induced skin injury are...

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Bibliographic Details
Published in:Processes 2021-09, Vol.9 (9), p.1564
Main Authors: Huang, Shang-Ming, Shieh, Chwen-Jen, Wu, Yu-Lin, Pan, Yang-Zing, Yu, Chi-Yang
Format: Article
Language:English
Subjects:
Acids
Antioxidants
Boiling
Cell viability
Chelation
Damage
Diabetes mellitus
DNA damage
Ethanol
Ferrous ions
Fibroblasts
Flavonoids
Free radicals
Herbal medicine
Herpes zoster
Inflammatory diseases
Irradiation
Phenolic compounds
Phenols
Potassium
Radiation
Reagents
Scavenging
Skin
Skin injuries
Spiranthes
Ultraviolet radiation
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Summary:Spiranthes sinensis (S. sinensis), a species of orchid, is a well-known herb medicine used to treat disorders such as stomachache, diabetes, shingles, and certain inflammatory diseases. Presently, the antioxidant activity of S. sinensis as well as its protective effect on UVB-induced skin injury are unclear. In this study, S. sinensis was extracted with boiling water or 75% (v/v) ethanol, and then its antioxidant composition and antioxidant activity were determined. The protective effects of S. sinensis against UVB-induced damage in human skin fibroblasts (CCD-966SK) were also investigated. Our data showed that the extraction yield of boiling water was higher than that of 75% ethanol. However, compared to the aqueous extracts, the ethanol extracts not only had higher phenolic, flavonoid, and condensed tannin contents, but also exhibited higher free radical scavenging activity, higher reducing power, and higher ferrous ion-chelating capacity. When fibroblasts were pre-cultured with the water or ethanol extracts of S. sinensis (1500 μg/mL) for 24 h before applying UVB irradiation, the S. sinensis extracts restored 17% to 27% of cell viability compared to a control only irradiated with UVB. Overall, our study suggests that S. sinensis extracts can be used as effective antioxidants and have the potential to protect skin fibroblasts from UVB irradiation.
ISSN:2227-9717
2227-9717
DOI:10.3390/pr9091564