First report of Hibiscus latent Singapore virus infecting hibiscus in Peninsular Malaysia

Evolutionary analysis by Maximum Likelihood method conducted by MEGA X. Phylogenetic trees generated from multiple sequence alignment of nucleotides with the highest percentage identity sequences. The evolutionary history was inferred by using the Maximum Likelihood method and the Tamura-Nei model T...

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Bibliographic Details
Published in:New disease reports 2021-07, Vol.44 (1), p.n/a
Main Authors: Yusop, M.S.M., Mohamed‐Hussein, Z.A., Bunawan, H.
Format: Article
Language:English
Subjects:
Maximum likelihood method
Nucleotide sequence
Phylogenetics
Phylogeny
Polymerase chain reaction
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Summary:Evolutionary analysis by Maximum Likelihood method conducted by MEGA X. Phylogenetic trees generated from multiple sequence alignment of nucleotides with the highest percentage identity sequences. The evolutionary history was inferred by using the Maximum Likelihood method and the Tamura-Nei model Total RNA was extracted from diseased leaves of 14 individual plants using a QIAGEN RNeasy Plant Mini Kit according to the manufacturer's protocol. RT-PCR products from the 14 samples were visualised in a 1% agarose gel and were the expected size (435 bp).
ISSN:2044-0588
2044-0588
DOI:10.1002/ndr2.12019