Role of the NO-cGMP-K+ channels pathway in the peripheral antinociception induced by α-bisabolol

• Published in: Canadian journal of physiology and pharmacology 2021-10, Vol.99 (10), p.1048-1056
• Main Authors: Ortiz, Mario I, Cariño-Cortés, Raquel, Muñoz-Pérez, Víctor M, Salas-Casas, Andrés, Castañeda-Hernández, Gilberto
• Format: Article
• Language: English
• Subjects: Analgesics - pharmacology; Animals; Arginine; canaux K; cGMP; Channel opening; Charybdotoxin; Cyclic GMP; Cyclic GMP - metabolism; Formaldehyde; Glibenclamide; GMPc; Injection; K+ channels; Male; Monocyclic Sesquiterpenes - pharmacology; Naloxone; Naltrexone; Narcotics; NG-Nitroarginine methyl ester; Nitric oxide; Nitric Oxide - metabolism; nociception; Nociception - drug effects; Opioid receptors; oxyde nitrique; Pain perception; Potassium Channel Blockers - pharmacology; Potassium channels; Potassium Channels - chemistry; Potassium Channels - genetics; Potassium Channels - metabolism; Rats; Rats, Wistar; Receptors, Opioid - chemistry; Receptors, Opioid - genetics; Receptors, Opioid - metabolism; Rodents; Tetraethylammonium; α-bisabolol
• ISSN: 0008-4212; 1205-7541
• DOI: 10.1139/cjpp-2020-0744

The aim of this study was to examine if the peripheral antinociception of α-bisabolol involves the participation of nitric oxide (NO) and cyclic guanosine monophosphate (cGMP) synthesis followed by K + channel opening in the formalin test. Wistar rats were injected in the dorsal surface of the right hind paw with formalin (1%). Rats received a subcutaneous injection into the dorsal surface of the paw of vehicles or increasing doses of α-bisabolol (100–300 µg/paw). To determine whether the peripheral antinociception induced by α-bisabolol was mediated by either the opioid receptors or the NO-cGMP-K + channels pathway, the effect of pretreatment (10 min before formalin injection) with the appropriate vehicles, naloxone, naltrexone, N G -nitro- l -arginine methyl ester (L-NAME), 1H-[1,2,4]-oxadiazolo[4,2-a]quinoxalin-1-one (ODQ), glibenclamide, glipizide, apamin, charybdotoxin, tetraethylammonium, or 4-aminopyridine on the antinociceptive effects induced by local peripheral α-bisabolol (300 µg/paw) were assessed. α-Bisabolol produced antinociception during both phases of the formalin test. α-Bisabolol antinociception was blocked by L-NAME, ODQ, and all the K + channels blockers. The peripheral antinociceptive effect produced by α-bisabolol was not blocked by the opioid receptor inhibitors. α-Bisabolol was able to active the NO-cGMP-K + channels pathway to produce its antinoceptive effect. The participation of opioid receptors in the peripheral local antinociception induced by α-bisabolol is excluded.