Effects of dietary active soybean trypsin inhibitors on pancreatic weights, histology and expression of STAT3 and receptors for androgen and estrogen in different tissues of rats

• Published in: Molecular biology reports 2021-05, Vol.48 (5), p.4591-4600
• Main Authors: Xiao, Chao-Wu, Wood, Carla, Cunningham, Lee Anne, Lalande, Maryline, Riding, Melissa
• Format: Article
• Language: English
• Subjects: Androgens; Animal Anatomy; Animal Biochemistry; Animals; Biomedical and Life Sciences; Casein; Diet; Estrogen Receptor alpha - genetics; Estrogen Receptor beta - genetics; Estrogen receptors; Estrogens; Estrogens - genetics; Female; Gene Expression Regulation - drug effects; Glycine max - chemistry; Histology; Hypertrophy; Life Sciences; Liver; Male; Morphology; Original Article; Pancreas; Pancreas - drug effects; Pancreas - metabolism; Proteins; Rats; Receptors, Androgen - genetics; Secretions; Soybean Proteins - genetics; Soybean Proteins - pharmacology; Soybeans; Stat3 protein; STAT3 Transcription Factor - genetics; Tissue Distribution - drug effects; Tissue Distribution - genetics; Trypsin; Trypsin Inhibitors - pharmacology; Trypsinogen; Uterus; Weights and Measures - standards
• ISSN: 0301-4851; 1573-4978
• DOI: 10.1007/s11033-021-06491-x

Our previous study showed that soy milks could contain high levels of active soybean trypsin inhibitors (SBTI) if they were not properly processed. This study investigated the effects of consuming active SBTI on pancreatic weights, histology, trypsinogen production and expression of STAT3, receptors for androgen (AR) and estrogen (ER) in pancreas, liver and uterus of rats. Weanling Sprague–Dawley rats were randomly divided into 3 groups (8 females and 8 males/group) and fed diets containing either 20% casein protein (Casein) or 20% soy protein (SP) in the presence of high (1.42 BAEE unit/µg, SP + SBTI) or low (0.2 BAEE unit/µg, SP-SBTI) levels of active SBTI for 8 weeks. Ingestion of SP + SBTI diet markedly increased pancreatic weights and trypsinogen content ( p