Mix-and-read, one-minute SARS-CoV-2 diagnostic assay: development of PIFE-based aptasensor

We developed a one-minute, one-step SARS-CoV-2 antigen assay based on protein-induced fluorescence enhancement of a DNA aptamer. The system showed significant selectivity and sensitivity towards both nucleocapsid protein and SARS-CoV-2 virus lysate, but with marked improvements in speed and manufact...

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Bibliographic Details
Published in:Chemical communications (Cambridge, England) England), 2021-10, Vol.57 (79), p.1222-1225
Main Authors: Lee, J. Michelle, Kim, Chae Rin, Kim, Sion, Min, Junhong, Lee, Min-Ho, Lee, SangWook
Format: Article
Language:English
Subjects:
Antigens
Fluorescence
Manufacturability
Proteins
Selectivity
Severe acute respiratory syndrome coronavirus 2
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Summary:We developed a one-minute, one-step SARS-CoV-2 antigen assay based on protein-induced fluorescence enhancement of a DNA aptamer. The system showed significant selectivity and sensitivity towards both nucleocapsid protein and SARS-CoV-2 virus lysate, but with marked improvements in speed and manufacturability. We hence propose this platform as a mix-and-read testing strategy for SARS-CoV-2 that can be applied to POC diagnostics in clinical settings, especially in low- and middle-income countries. Here, we developed a rapid and quantitative one-step severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) antigen assay based on protein-induced fluorescence enhancement (PIFE) of a nucleocapsid (N) protein DNA aptamer.
ISSN:1359-7345
1364-548X
DOI:10.1039/d1cc04066a