Mix-and-read, one-minute SARS-CoV-2 diagnostic assay: development of PIFE-based aptasensor

We developed a one-minute, one-step SARS-CoV-2 antigen assay based on protein-induced fluorescence enhancement of a DNA aptamer. The system showed significant selectivity and sensitivity towards both nucleocapsid protein and SARS-CoV-2 virus lysate, but with marked improvements in speed and manufact...

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Published in:Chemical communications (Cambridge, England) England), 2021-10, Vol.57 (79), p.1222-1225
Main Authors: Lee, J. Michelle, Kim, Chae Rin, Kim, Sion, Min, Junhong, Lee, Min-Ho, Lee, SangWook
Format: Article
Language:English
Subjects:
Antigens
Fluorescence
Manufacturability
Proteins
Selectivity
Severe acute respiratory syndrome coronavirus 2
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container_title Chemical communications (Cambridge, England)
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creator Lee, J. Michelle
Kim, Chae Rin
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Lee, Min-Ho
Lee, SangWook
description We developed a one-minute, one-step SARS-CoV-2 antigen assay based on protein-induced fluorescence enhancement of a DNA aptamer. The system showed significant selectivity and sensitivity towards both nucleocapsid protein and SARS-CoV-2 virus lysate, but with marked improvements in speed and manufacturability. We hence propose this platform as a mix-and-read testing strategy for SARS-CoV-2 that can be applied to POC diagnostics in clinical settings, especially in low- and middle-income countries. Here, we developed a rapid and quantitative one-step severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) antigen assay based on protein-induced fluorescence enhancement (PIFE) of a nucleocapsid (N) protein DNA aptamer.
doi_str_mv 10.1039/d1cc04066a
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subjects Antigens
Fluorescence
Manufacturability
Proteins
Selectivity
Severe acute respiratory syndrome coronavirus 2
title Mix-and-read, one-minute SARS-CoV-2 diagnostic assay: development of PIFE-based aptasensor
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