Influence of Chlorides of Mono- and Divalent Metals on the Oligomeric Composition of Lysozyme Crystallization Solutions and Further Crystal Growth

The influence of the precipitant type (LiCl, NaCl, KCl, NiCl 2 , and CuCl 2 ) on the formation of oligomers (dimers and octamers) in lysozyme crystallization solutions at two protein concentrations has been investigated by small-angle X-ray scattering (SAXS). The same solutions have been used to gro...

Full description

Saved in:
Bibliographic Details
Published in:Crystallography reports 2021-09, Vol.66 (5), p.751-757
Main Authors: Marchenkova, M. A., Konarev, P. V., Boikova, A. S., Ilina, K. B., Pisarevsky, Yu. V., Kovalchuk, M. V.
Format: Article
Language:English
Subjects:
Atomic properties
Copper chloride
Crystal growth
Crystallization
Crystallography and Scattering Methods
Crystals
Diffraction and Scattering of Ionizing Radiations
Dimers
Lithium
Lysozyme
Oligomers
Physics
Physics and Astronomy
Proteins
Sodium
X-ray scattering
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The influence of the precipitant type (LiCl, NaCl, KCl, NiCl 2 , and CuCl 2 ) on the formation of oligomers (dimers and octamers) in lysozyme crystallization solutions at two protein concentrations has been investigated by small-angle X-ray scattering (SAXS). The same solutions have been used to grow crystals in order to reveal the influence of the oligomeric composition on the crystal growth. The data obtained in this and previous studies on the influence of precipitant concentration yield an inversely proportional dependence of the total content of octamers and dimers on the cation atomic number, which is in agreement with the increase in the ion activity in the lyotropic series for Li + , Na + , and K + and the increase in the ionic radius for Li + , Na + , K + , Ni 2+ , and Cu 2+ . It is shown that a decrease in the protein concentration in a crystallization solution leads to a decrease in octamer volume fraction at an invariable volume fraction of dimers and reduces the probability of crystal formation.
ISSN:1063-7745
1562-689X
DOI:10.1134/S1063774521050138