SlNCED1 affects pollen maturation in tomato by regulating the expression of anther-specific genes

The plant hormone abscisic acid (ABA) plays an important role in many aspects of plant growth and development, and a growing number of evidences show that ABA may play an important role in the development of tomato pollen development. To further analyze the role of ABA in the development of tomato p...

Full description

Saved in:
Bibliographic Details
Published in:Plant growth regulation 2021-11, Vol.95 (2), p.191-205
Main Authors: Wang, Xiaohua, Xu, Na, Dong, Kai, Li, Hao, Shi, Shaokun, Liu, Zhenning, Cao, Xue, Cui, Xiaoyu, Dai, Shengjie
Format: Article
Language:English
Subjects:
Abscisic acid
Agriculture
Biomedical and Life Sciences
Biosynthesis
Dioxygenase
Gene expression
Gene regulation
Genes
Genetic transformation
Life Sciences
Maturation
Original Paper
Plant Anatomy/Development
Plant growth
Plant hormones
Plant Physiology
Plant Sciences
Pollen
RNA-mediated interference
Tomatoes
Transcription
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The plant hormone abscisic acid (ABA) plays an important role in many aspects of plant growth and development, and a growing number of evidences show that ABA may play an important role in the development of tomato pollen development. To further analyze the role of ABA in the development of tomato pollen, the SlNCED1 gene encoding 9-cis-epoxycarotenoid dioxygenase (NCED), a key enzyme in the ABA biosynthesis, was suppressed in tomato plants by transformation with RNA interference (RNAi). Endogenous ABA content and SlNCED1 transcript levels in the transgenic anther were down-regulated to between 20 and 55% of the levels measured in the wild-type anther. The significant reduction of NCED activity led to a down-regulation in the transcription of specific genes positively related to anther development in tomato, such as TomA92b9 , TomA108 , SlbHLH080 and 5B-CRP . However, leading to an up-regulation in the transcription of specific genes negatively related to anther development in tomato, such as adh-3a , LAT58 , LePro1 and SlPMEI . This resulted in abnormal anther development, which led to the development of a large number of abnormal pollens. In conclusion, ABA affects pollen maturation by regulating the expression of anther-specific genes.
ISSN:0167-6903
1573-5087
DOI:10.1007/s10725-021-00732-6