Fast blue B functionalized silica-polymer composite to evaluate 3,5-dihydroxyhydrocinnamic acid as biomarker of gluten intake

• Published in: Sensors and actuators. B, Chemical Chemical, 2021-10, Vol.345, p.130333, Article 130333
• Main Authors: Hakobyan, L., Prieto-Blanco, M.C., Llorens, María Roca, Molins-Legua, C., Fuster-Garcia, M., Moliner-Martinez, Y., Campins-Falcó, P., Ribes-Koninckx, Carmen
• Format: Article
• Language: English
• Subjects: alkylresorcinols; Autoimmune diseases; Biological properties; Biomarkers; capillary liquid chromatography; Capillary tubes; celiac disease; Chromatography; Colorimetry; Diet; fast blue B; Gluten; Low concentrations; PDMS composites; Physicians; Polydimethylsiloxane; Polymer matrix composites; Polymers; Prolamines; Reagents; Silicon dioxide
• ISSN: 0925-4005; 1873-3077
• DOI: 10.1016/j.snb.2021.130333

[Display omitted] •Alkylresorcinols as biomarkers of gluten intake.•DHCA as target analyte in urine samples.•FB-doped PDMS-TEOS membranes as colorimetric sensing devices.•UV-vis spectroscopic at 520 nm for screening negative/positive responses.•In-tube SPME-CapLC to confirm DHCA as biomarker of gluten in-take. Celiac disease is an immune-mediated systemic disorder elicited by gluten and related prolamines in genetically susceptible individuals. The current treatment is a strict and lifelong gluten-free diet. However, compliance with the gluten-free diet is not always adequate and many food products contain low concentrations of gluten. The determination of dietary transgressions is a challenge for patients, physicians and dietitians. Alkylresorcinols (AR) have been proposed as sensitive and specific biomarkers of gluten consumption. In this work silica–polymer composites doped with fast blue B reagent (FB) have been used to estimate alkylresorcinols in biological samples. The proposed colorimetric device was synthetized by immobilizing FB into polydimethylsiloxane-tetraethylortosilicate composite. The assay was based on the spontaneous release of FB to the solution containing AR (3,5-dihydroxyhydrocinnamic acid, DHCA) and the azocomplexe formation (520 nm). The response was evaluated with UV-vis spectroscopy and chromatography (in-tube SPME-Capillary LC-DAD) to isolate DHCA signal. The spectroscopic analysis can be used as a screening tool to differentiate positive and negative samples. Meanwhile, the chromatographic assay was necessary to isolate DHCA response in positive samples. LOD was 60 ng mL -1 by adding a SPE step. Precision provided adequate results (RSD