Mapping and mining of major genomic regions conferring resistance to Bruchine (Callosobruchus maculatus) in blackgram (Vigna mungo)

Storage pest bruchine is a major production limiting factor in blackgram. The present study was employed in a bi‐parental mapping population derived from a cross between susceptible (MDU 1) and resistant (TU68) genotypes. Phenotyping was carried out under an artificial bruchine screening procedure....

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Bibliographic Details
Published in:Plant breeding 2021-10, Vol.140 (5), p.896-906
Main Authors: Subramaniyan, Ragul, Narayana, Manivannan, Krishnamoorthy, Iyanar, Natarajan, Ganapathy, Gandhi, Karthikeyan
Format: Article
Language:English
Subjects:
blackgram
Breeding
bruchine resistance
candidate genes
Gene mapping
Genotypes
Herbivory
Insects
Kinases
Leucine
Mapping
Markers
Pest resistance
Phenotyping
Protein kinase
Protein-serine/threonine kinase
Proteins
Quantitative trait loci
seed damage
SSR
Transcription factors
Zinc finger proteins
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Summary:Storage pest bruchine is a major production limiting factor in blackgram. The present study was employed in a bi‐parental mapping population derived from a cross between susceptible (MDU 1) and resistant (TU68) genotypes. Phenotyping was carried out under an artificial bruchine screening procedure. QTL studies revealed three major QTLs responsible for the total number of adult emergence, percentage of seed damage and developmental time. The validation of identified QTLs was carried out on other mapping populations, namely, VBN 6 × TU 68 and VBN 8 × TU 68 through single marker analysis. It revealed that markers CEDG020 and CEDG302 can be used in the future bruchine resistance breeding programme. Genome mining on the QTL regions harbours several possible candidate genes related to defence response against herbivory insects, namely, serine/threonine protein kinase proteins, zinc finger family proteins, F‐box proteins, leucine‐rich repeats regions and some transcription factors and enzymes. The outcome of this study will be useful for bruchine resistance breeding programme.
ISSN:0179-9541
1439-0523
DOI:10.1111/pbr.12959