AuPt nanocrystals/polydopamine supported on open-pored hollow carbon nanospheres for a dual-signaling electrochemical ratiometric immunosensor towards h-FABP detection

[Display omitted] •AuPt/PDA/OHCSs had the large specific surface area and high porosity for efficient adsorption of Thi.•The Au NDs were linked with Chit-g-Fc to provide stable signals upon attachment of the antibodies.•The multi-signal output largely enhanced the sensitivity of the biosensor.•The r...

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Published in:Sensors and actuators. B, Chemical Chemical, 2021-11, Vol.346, p.130501, Article 130501
Main Authors: Feng, Yi-Ge, Zhu, Jian-Hong, Wang, Ai-Jun, Mei, Li-Ping, Luo, Xiliang, Feng, Jiu-Ju
Format: Article
Language:English
Subjects:
Biomarkers
Carbon
Chitosan
Fatty acids
Gold
Heart-type fatty acid-binding protein
Immunosensors
Multi-signal output
Nanocrystals
Nanodendrites
Nanospheres
Open-pored hollow carbon spheres
Ratiometric immunoassay
Substrates
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Summary:[Display omitted] •AuPt/PDA/OHCSs had the large specific surface area and high porosity for efficient adsorption of Thi.•The Au NDs were linked with Chit-g-Fc to provide stable signals upon attachment of the antibodies.•The multi-signal output largely enhanced the sensitivity of the biosensor.•The ratiometric immunoassay showed excellent analytical performance for h-FABP. For early diagnosis and prevention of acute myocardial infarction (AMI), accurate and sensitive analysis of cardiac biomarkers by a single test has faced substantial challenge. A novel electrochemical immuno-sensing platform was built for untrasensitive assay of heart-type fatty acid-binding protein (h-FABP) via multi-signal output. This immunosensor utilized Au nanodendrites/chitosan-grafted-ferrocene (Au NDs/Chit-g-Fc) as the substrate and thionine-absorbed AuPt nanocrystals/polydopamine/open-pored hollow carbon spheres (Thi/AuPt/PDA/OHCSs) as the label material, producing two independent electrochemical signals from the Fc and Thi probes, respectively. For such assay of the target h-FABP in the immuno-sensing system, the ratios (IThi / IFc) between the double signals showed a broad dynamic range of 0.001–200.0 ng mL−1 and a low detection limit of 0.53 pg mL−1 (S/N = 3). This ratiometric sensor exerts a far-reaching influence on other biomarkers researches in practical applications.
ISSN:0925-4005
1873-3077
DOI:10.1016/j.snb.2021.130501