Simple and sensitive diagnosis of invasive aspergillosis using triphasic DE−ZnO−APDMS microparticle composite

•A simple and sensitive diagnosis of invasive aspergillosis.•A novel triphasic DE-ZnO-APDMS microparticle composite.•The microparticles used for fungal spores enrichment and fungal cell wall disintegration without detergents.•Validated the system in 30 clinical samples showed superior sensitivity (1...

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Bibliographic Details
Published in:Sensors and actuators. B, Chemical Chemical, 2021-11, Vol.346, p.130487, Article 130487
Main Authors: Qiao, Zhen, Seo, Hyeonji, Liu, Huifang, Cha, Hye-Hee, Kim, Ji Yeun, Kim, Sung-Han, Shin, Yong
Format: Article
Language:English
Subjects:
Assaying
Detergents
Diagnosis
Diatomaceous earth
Disintegration
Fungal enrichment
Fungi
Invasive aspergillosis
Microparticles
Molecular diagnostics
Rapid diagnostics
Spores
Zinc oxide
Zinc oxides
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Summary:•A simple and sensitive diagnosis of invasive aspergillosis.•A novel triphasic DE-ZnO-APDMS microparticle composite.•The microparticles used for fungal spores enrichment and fungal cell wall disintegration without detergents.•Validated the system in 30 clinical samples showed superior sensitivity (100 %) to the conventional kit (50 %). The increasing mortality and incidence of invasive aspergillosis (IA) pose a serious threat to the health of immunocompromised populations. Herein, we synthesized triphasic microparticles named diatomaceous earth‒zinc oxide‒3-aminopropyl(diethoxy)methylsilane (DE‒ZnO‒APDMS) for spores enrichment and fungal cell wall disintegration without detergents when incubated with Aspergillus fumigatus (A. fumigatus). Using the triphasic DE‒ZnO‒APDMS microparticles and PCR assays, we developed a simple and sensitive A. fumigatus diagnostic assay. In tests with cultured A. fumigatus samples, the diagnosis assay we presented has shown its superiority over commercial kit, including the ease of fungal cell wall disintegration, spores capture, and the isolation of fungal DNA during sample preparation. Furthermore, the clinical utility of the proposed fungal diagnostic assay in 30 clinical samples showed superior sensitivity (100 %) to the conventional kit (50 %). The developed DE‒ZnO‒APDMS-based fungal diagnostic assay is a potentially valuable tool for diagnosing and monitoring invasive aspergillosis.
ISSN:0925-4005
1873-3077
DOI:10.1016/j.snb.2021.130487