Chemical composition and vasodilator activity of different Alpinia zerumbet leaf extracts, a potential source of bioactive flavonoids

A polyphenol-rich extract with an expressive vasodilator effect was obtained from fresh leaves of Alpinia zerumbet (AZ), a medicinal plant. The vasodilator effects of AZ from different extraction conditions were studied in the perfused mesenteric vascular bed, and total polyphenol content (TPC) was...

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Bibliographic Details
Published in:Medicinal chemistry research 2021-11, Vol.30 (11), p.2103-2113
Main Authors: da Silva, Munyck A., de Carvalho, Lenize C. R. M., Victório, Cristiane P., Ognibene, Dayane T., Resende, Angela C., de Souza, Marcelo A. V.
Format: Article
Language:English
Subjects:
Alpinia zerumbet
Biochemistry
Biological activity
Biomedical and Life Sciences
Biomedicine
Bioorganic Chemistry
Cardiovascular diseases
Chemical composition
Epicatechin
Flavonoids
Heating
Herbal medicine
Inorganic Chemistry
Kaempferol
Leaves
Medicinal Chemistry
Medicinal plants
Natural products
Original Research
Pharmacology/Toxicology
Plant extracts
Social organization
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Summary:A polyphenol-rich extract with an expressive vasodilator effect was obtained from fresh leaves of Alpinia zerumbet (AZ), a medicinal plant. The vasodilator effects of AZ from different extraction conditions were studied in the perfused mesenteric vascular bed, and total polyphenol content (TPC) was determined for all samples. UHPLC/ESI-QTOF-MS analysis established the chemical composition of AZ extracts, and a molecular network from GNPS (Global Natural Products Social Molecular Networking) generated a list of putative compounds. Briefly, results show a 50% hydroethanolic extract from fresh leaves (AZE4) as the best extraction condition, presenting the greatest vasodilator effect (ED 50  = 7.1 ± 1.7 µg) and TPC (16.30 mg GAE/g ± 0.44). Flavonoids are the main constituents and alpinetin, kaempferol-3- O -glucuronide, (−)-epicatechin, and pinocembrin were identified as major compounds. The least effective extraction condition was 50% hydroethanolic extract from dried leaves without heating. This extract showed the smallest vasodilator response (ED 50  = 29.1 ± 4.3 µg) and TPC (6.35 mg GAE/g ± 0.08), reinforcing the use of fresh leaves and heating step to improve extracts performance. The vasodilator effect and the main flavonoid composition of AZE4 provide experimental support, suggesting this extract as a potential source of bioactive flavonoids to treat cardiovascular diseases.
ISSN:1054-2523
1554-8120
DOI:10.1007/s00044-021-02791-w