Skirmishing MDR strain of Candida albicans by effective antifungal CeO2 nanostructures using Aspergillus terreus and Talaromyces purpurogenus

Emerging antibiotics resistance fungal infectionsis a major global health problem and new antifungal formulations are direly needed to fight drug resistant Candida albicans strains. This study is aimed to synthesize effective antifungal nanostructures of cerium oxide (CeO2) using culture filtrates o...

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Bibliographic Details
Published in:Materials research express 2020-05, Vol.7 (5), p.055004
Main Authors: Komal, Rimsha, Uzair, Bushra, Sajjad, Shamaila, Butt, Sajid, Kanwal, Amna, Ahmed, Iftikhar, Riaz, Naveeda, Leghari, Sajjad Ahmed Khan, Abbas, Sehrish
Format: Article
Language:English
Subjects:
anti fungal activity
Antibiotics
Aspergillus terreus
Candida albicans
cerium oxide nanoparticles
Cerium oxides
Ethanol
Fourier transforms
Fungi
Fungicides
Gas chromatography
Gene sequencing
Mass spectrometry
Methyl chloride
Morphology
Nanoparticles
Nanostructure
Palmitic acid
Public health
Synthesis
Talaromyces purpureogenus
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Summary:Emerging antibiotics resistance fungal infectionsis a major global health problem and new antifungal formulations are direly needed to fight drug resistant Candida albicans strains. This study is aimed to synthesize effective antifungal nanostructures of cerium oxide (CeO2) using culture filtrates of two common fungal strains Aspergillus terreus and Talaromyces pupureogenus. The fungal strains used in the synthesis were identified by 18S rRNA gene sequencing and deposited to NCBI GenBank with the accession number of MN099077 and MN121629, respectively. The biofabricated CeO2 NPs were characterized by X-ray diffraction (XRD), Fourier-transform infrared spectroscopy (FTIR) and Scanning Electron Microscopy (SEM). Pure CeO2 nanoparticles (NPs) synthesized using Aspergillus terreus culture filtrate were depicted spherical morphology with average size of 28.5 nm. The CeO2NPs synthesized using Talaromyces pupureogenus revealed the presence of nanosponges with average size of 21.4 nm. Gas chromatography mass spectrometry of culture filtrates of respective strains indicated the presence of ethanol, 1-propanol and tri-chloromethane in culture filtrate of Aspergillus terreus and with addition of palmitic acid in Talaromyces pupureogenus culture filtrate which may have a function as bio reducers and capping agents. Dose dependent anticandidal activity of CeO2 NPs using various different concentrations (100, 200, 300, 600 g ml−1) synthesized by both fungal strains was observed by disc diffusion assay against Candida albicansas evidenced by increase in size of zone of inhibitions with increasing concentration of CeO2NPs. Further in-vitro and in-vivo experiments are required to access the potential of CeO2 NPs for controlling Candida albicans strains.
ISSN:2053-1591
DOI:10.1088/2053-1591/ab8ba2